+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The spleen focus-forming virus (SFFV) envelope gene, when introduced into mice in the absence of other SFFV genes, induces acute erythroleukemia



The spleen focus-forming virus (SFFV) envelope gene, when introduced into mice in the absence of other SFFV genes, induces acute erythroleukemia



Journal of Virology 62(6): 2158-2163



Previous studies in our laboratory and others have been consistent with the hypothesis that the envelope (env) gene of the spleen focus-forming virus (SFFV) is the only gene essential for the induction of acute erythroleukemia. However, no studies have been carried out with the SFFV env gene in the complete absence of other SFFV sequences. To accomplish this goal, we isolated the sequences that encode the envelope glycoprotein, gp52, of SFFVA and expressed them in a Moloney murine leukemia virus-based double-expression vector containing the neomycin resistance gene. The method used to produce retrovirus stocks in tissue culture cells affected the expression of the gp52 gene in the vector and the subsequent pathogenicity of the vector in mice. Highly pathogenic virus stocks were obtained by cotransfection of vector and helper virus DNAs into fibroblasts, followed by virus replication and spread through the cell population. Mice infected with this stock developed a rapid erythroid disease that was indistinguishable from that induced by the entire SFFV genome, and the virus stock transformed erythroid cells in vitro. Spleen cells from the diseased mice expressed the SFFV env gene product but not the SFFV gag gene product. As expected, mice given the virus containing the SFFV env gene in the reverse orientation did not express the SFFV env gene product or develop erythroleukemia. This study, therefore, demonstrated (i) that double-expression retroviral vectors can be used under specific conditions to produce viruses expressing high levels of a particular gene and (ii) that incorporation of the SFFV env gene into such a vector in the absence of other SFFV sequences results in a retrovirus which is as pathogenic as the original SFFV.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 041758793

Download citation: RISBibTeXText

PMID: 2835516


Related references

A 585-bp deletion found in the spleen focus-forming virus (SFFV) env gene is responsible for the defective intracellular transport of SFFV gp52. Virology 188(1): 181-192, 1992

A new series of RNAs associated with the genome of spleen focus forming virus (SFFV) and poly(A)-containing RNA from SFFV-infected cells. Nucleic Acids Research 7(4): 895-908, 1979

Structural and functional studies of the Friend spleen focus-forming virus: structural relationship of SFFV to dualtropic viruses and molecular cloning of a biologically active subgenomic fragment of SFFV DNA. Haematology and Blood Transfusion 26: 472-478, 1981

A new series of Rnas associated with the genome of spleen focus fonning virus (Sffv) and poly(A)-containing Rna from Sffv-infected cells. Nucleic Acids Research 7(4): 895-908, 1979

Sequences responsible for the altered erythropoietin responsiveness in spleen focus forming virus strain sffv p infected cells are localized to a 678 base pair region at the 3' end of the envelope gene. Journal of Virology 61(5): 1661-1664, 1987

A carboxy-terminal mutant spleen focus-forming virus (SFFV) envelope glycoprotein is transport-competent, but non-leukemogenic. Virus Research 26(1): 57-69, 1992

Oncogene cooperativity in Friend erythroleukemia: Erythropoietin receptor activation by the env gene of SFFV leads to transcriptional upregulation of PU.1, independent of SFFV proviral insertion. Oncogene 21(8): 1272-1284, 14 February, 2002

Transport and leukemogenicity of spleen focus forming virus sffv glycoproteins. Journal of Cell Biology 111(5 Part 2): 103A, 1990

Oligomerization of Friend spleen focus-forming virus (SFFV) env glycoproteins. Virology 177(1): 312-316, 1990

Identification of Proteins Specific to Friend Strain of Spleen Focus forming Virus (SFFV). Proceedings of the Japan Academy Series B Physical and Biological Sciences 54(10): 651-656, 1978

B-tropic Friend virus: a host-range pseudotype of spleen focus-forming virus (SFFV). Virology 55(2): 363-370, 1973

New U1 RNA species found in Friend SFFV (spleen focus forming virus)-transformed mouse cells. Journal of Biological Chemistry 260(12): 7775-7782, 1985

Viral targeting of genes which cooperate with sffv gp55 gene for erythroleukemia development in transgenic mice. Proceedings of the American Association for Cancer Research Annual Meeting 33: 376, 1992

Molecular analysis of the cell type specific mitogenic activity of the env like glycoprotein gp55 of friend spleen focus forming virus sffv. Nishizuka, Y , M Endo And C Tanaka (Ed ) Advances in Second Messenger And Phosphoprotein Research, Vol 24 The Biology And Medicine Of Signal Transduction; 7th International Conference on Cyclic Nucleotides, Calcium And Protein Phosphorylation, Kobe, Japan, October 8-13, 1989 Xxxiii+750p Raven Press: New York, New York, Usa Illus 702, 1990

Mutational analysis of the structure-function relationship of the leukemogenic membrane glycoprotein (GP55) of Friend spleen focus-forming virus (F-SFFV). Leukemia 11(Suppl. 3): 160-161, 1997