+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Thermally-induced delayed fluorescence of photosystem I and II chlorophyll in thermophilic cyanobacterium Synechococcus elongatus



Thermally-induced delayed fluorescence of photosystem I and II chlorophyll in thermophilic cyanobacterium Synechococcus elongatus



General Physiology and Biophysics 11(3): 229-239



Stationary delayed fluorescence (DF) of chlorophyll in isolated membrane preparations from thermophilic cyanobacterium Synechococcus elongatus was investigated as a function of temperature. Two peaks at different temperatures were observed. The low-temperature peak (54-60 degrees C) coincided with the main maximum of the thermally-induced delayed fluorescence of chlorophyll in intact cells and PSII-particles with active oxygen-evolving system. The high-temperature peak (78 degrees C) coincided with the minor band of delayed light emitted by intact cells. It was also observed in the delayed fluorescence emission from a PSI-enriched fraction preparation. The intensities of the DF peaks were dependent on the presence of inhibitors, donors and acceptors that cause specific effects on electron transport of the two photosystems. The low-temperature and high-temperature peaks were related to PSII and PSI, respectively. The manifestation of delayed fluorescence from PSI and PSII at different temperatures seems to be a specific property of thermophilic cyanobacteria. The reason for this may be a high thermal stability of the photosystems and the lack of the PSII antenna complex in isolated membranes. Consequently, the relative yield of delayed fluorescence from PSI markedly increases. Thermally-induced fluorescence seen in membranes of cyanobacteria showed a high sensitivity to structural and functional membrane alterations induced by pH changes, different electron transport stabilizing agents or different concentrations of MgCl2.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 041796272

Download citation: RISBibTeXText

PMID: 1426973


Related references

Thermo induced delayed fluorescence of chlorophyll of photosystems i and ii from membranes of the thermophilic cyanobacterium synechococcus elongatus. Biologicheskie Membrany 5(12): 1289-1296, 1988

N-terminal amino acid sequence of the chlorophyll-binding protein CP-47 of Photosystem 2 in the thermophilic cyanobacterium Synechococcus elongatus. Bioscience Biotechnology and Biochemistry 56(2): 328-329, 1992

Functional analysis of PSII-T protein of photosystem II complex from the thermophilic cyanobacterium Synechococcus elongatus. Plant & Cell Physiology 42(Suppl.): s170, 2001

Interaction of benzoquinones with QA- and QB- in oxygen-evolving photosystem II particles from the thermophilic cyanobacterium Synechococcus elongatus. Plant and Cell Physiology 31: 39-47, 1990

Molecular weight determination of an active photosystem I preparation from a thermophilic cyanobacterium, Synechococcus elongatus. Biochemistry 29(5): 1216-1225, 1990

Integrity and activity of photosystem 2 complexes isolated from the thermophilic cyanobacterium Synechococcus elongatus using various detergents. Photosynthetica 37(2): 183-200, 1999

Secondary structure and thermostability of the photosystem II manganese-stabilizing protein of the thermophilic cyanobacterium Synechococcus elongatus. Biochimica et Biophysica Acta 1297(2): 167-170, 1996

Targeted disruption of psbX and biochemical characterization of photosystem II complex in the thermophilic cyanobacterium Synechococcus elongatus. Plant and Cell Physiology 42(2): 179-188, 2001

Amino acid sequence of the 8-kDa protein in photosystem I reaction center complex from a thermophilic cyanobacterium, Synechococcus elongatus. Protein Sequences and Data Analysis 4(6): 327-331, 1991

Preparation and characterization of heat stable and very active oxygen evolving photosystem ii particles from the thermophilic cyanobacterium synechococcus elongatus. Plant & Cell Physiology 33(3): 299-305, 1992

Amino acid sequence of 10-kDa protein in photosystem I reaction-center complex from a thermophilic cyanobacterium, Synechococcus elongatus Naegeli. Protein Sequences and Data Analysis 4(2): 93-96, 1991

Immunocytochemical localization of IdiA, a protein expressed under iron or manganese limitation in the mesophilic cyanobacterium Synechococcus PCC 6301 and the thermophilic cyanobacterium Synechococcus elongatus. Planta 205(1): 73-81, 1998

Heat stability of iron sulfur centers and p 700 in photosystem i reaction center complexes isolated from the thermophilic cyanobacterium synechococcus elongatus. Plant & Cell Physiology 31(6): 865-870, 1990