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Thin-layer chromatography/bioautography method for detection of monensin in poultry tissues

Thin-layer chromatography/bioautography method for detection of monensin in poultry tissues

Journal - Association of Official Analytical Chemists 72(5): 735-738

ISSN/ISBN: 0004-5756

PMID: 2808232

Monensin is an effective anticoccidial agent widely used in the poultry industry. Because of concerns over its toxicity, a sensitive, reliable, fast, and simple method for residue detection in poultry tissues is desirable from both a diagnostic and a regulatory view. Many methods of detection are excluded due to this ionophore's complex chemical and biological nature. A common method used for its detection is thin-layer chromatography/bioautography (TLC/B), although this is usually only semiquantitative. A new TLC/B method for monensin detection in poultry tissues was developed and is reported here. Recovery from cardiac muscle, skeletal muscle, and liver and kidney tissues is in the range of 93-97%. A detection limit of 250 micrograms/kg with 99% reliability was achieved with this method. Lower limits (to 10 micrograms/kg) were detectable, but with lower reliability (60%). Quantitative analysis is not possible on samples from fatty tissue.

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Accession: 041798391

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Thin-layer chromatography/bioautography method for detection of monensin in poultry tissues - Une méthode de chromatographie en couche mince/bioautographie pour la détection de la monensine dans les tissus de volaille. Journal - Association of Official Analytical Chemists 72(5): 735-738, 1989

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