EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Thin-layer chromatography/bioautography method for detection of monensin in poultry tissues


Thin-layer chromatography/bioautography method for detection of monensin in poultry tissues



Journal - Association of Official Analytical Chemists 72(5): 735-738



ISSN/ISBN: 0004-5756

PMID: 2808232

Monensin is an effective anticoccidial agent widely used in the poultry industry. Because of concerns over its toxicity, a sensitive, reliable, fast, and simple method for residue detection in poultry tissues is desirable from both a diagnostic and a regulatory view. Many methods of detection are excluded due to this ionophore's complex chemical and biological nature. A common method used for its detection is thin-layer chromatography/bioautography (TLC/B), although this is usually only semiquantitative. A new TLC/B method for monensin detection in poultry tissues was developed and is reported here. Recovery from cardiac muscle, skeletal muscle, and liver and kidney tissues is in the range of 93-97%. A detection limit of 250 micrograms/kg with 99% reliability was achieved with this method. Lower limits (to 10 micrograms/kg) were detectable, but with lower reliability (60%). Quantitative analysis is not possible on samples from fatty tissue.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 041798391

Download citation: RISBibTeXText

Related references

Thin-layer chromatography/bioautography method for detection of monensin in poultry tissues - Une méthode de chromatographie en couche mince/bioautographie pour la détection de la monensine dans les tissus de volaille. Journal - Association of Official Analytical Chemists 72(5): 735-738, 1989

Separation and detection of monensin, lasalocid and salinomycin by thin-layer chromatography/bioautography. Journal of Chromatography 508(2): 386-390, 1990

Investigation of monensin residues in chicken and beef by thin layer chromatography and bioautography method. Journal of the Chinese Agricultural Chemical Society 32(5): 497-506, 1994

Détection et identification du monensin, du narasin, du lasalocide, de la salinomycine et de la maduramicine en alimentation animale par chromatographie couche mince et bioautographie - Detection and identification of monensin, narasin, lasalocid, salinomycin and maduramicin in animal feeds and premixtures by thin-layer chromatography and bioautography. Journee Scientifique de la Section Est de la Societe des Experts Chimistes de France 93(950): 83-93, 2000

Determination of the disappearance rate and residual levels of monensin in chick tissues by quantitative thin layer bioautography. Journal of the Food Hygienic Society of Japan 21(3): 177-183, 1980

MATRIX SOLID-PHASE DISPERSION COMBINED WITH THIN-LAYER CHROMATOGRAPHY-DIRECT BIOAUTOGRAPHY FOR DETERMINATION OF FLUMEQUINE RESIDUES IN MILK: IMPROVEMENT OF THE METHOD : Thin layer chromatography. Journal of Liquid Chromatography and Related Technologies 34(9-12): 920-927, 2011

Simultaneous detection of the antibiotics monensin, salinomycin, narasin and lasalocid in poultry meat by thin-layer chromatography with bioautographic assay. Wiener Tierärztliche Monatsschrift 79(12): 365-369, 1992

Bioautography detection in thin-layer chromatography. Journal of Chromatography. a 1218(19): 2684-2691, 2011

Determination of monensin in poultry meat by thin-layer chromatography. Roczniki Panstwowego Zakladu Higieny 34(3): 281-283, 1983

Chemical classification of antibiotics in meat by the disc method and by thin layer chromatography bioautography. Journal of the Food Hygienic Society of Japan 22(4): 299-306, 1981

Identification and semiquantitation of monensin sodium in animal feeds by thin layer bioautography. Journal - Association of Official Analytical Chemists 66(6): 1506-1509, 1983

Thin-layer chromatography: Direct bioautography as a method of examination of antimicrobial activity of selected Potentilla species. Journal of Liquid Chromatography and Related Technologies 39(5-6): 281-285, 2016

Thin-layer chromatography-direct bioautography as an alternative method for screening of antibiotic residues in milk: A comparative study. Journal of Liquid Chromatography and Related Technologies 39(5-6): 292-297, 2016

Thin-layer chromatography-direct bioautography of flumequine residues in milk : This layer chromatography. Journal of Liquid Chromatography and Related Technologies 29(13-16): 2083-2093, 2006

Isolation of antioxidative compounds from Micromelum minutum guided by preparative thin layer chromatography-2,2-diphenyl-1-picrylhydrazyl (PTLC-DPPH) bioautography method. Food Chemistry 272: 185-191, 2019