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Thin-layer isoelectric focusing of hemoglobin variants: screening and determination of isoelectric points

Giuliani, A.; Marinucci, M.; Cappabianca, M.P.; Maffi, D.; Tentori, L.

Clinica Chimica Acta; International Journal of Clinical Chemistry 90(1): 19-28

1978

ISSN/ISBN: 0009-8981
PMID: 719888
DOI: 10.1016/0009-8981(78)90079-7
Accession: 041798403
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The high resolving power of thin-layer isoelectric focusing was applied for screening some hemoglobin variants classified on the basis of their electrophoretic mobility in: electrophoretically slow variants (as Hb A2), electrophoretically slow variants (as Hb S), electrophorectically fast variants (Hbs type J). An analysis of the variant compounds has been performed, and the corresponding pI values were determined in whole hemolysate.

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