+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Three intergenic regions of coronavirus mouse hepatitis virus strain A59 genome RNA contain a common nucleotide sequence that is homologous to the 3' end of the viral mRNA leader sequence



Three intergenic regions of coronavirus mouse hepatitis virus strain A59 genome RNA contain a common nucleotide sequence that is homologous to the 3' end of the viral mRNA leader sequence



Journal of Virology 53(3): 834-840



cDNA clones that represent various portions of the coronavirus mouse hepatitis virus strain A59 genome RNA have been constructed. cDNAs were synthesized by transcription of genome RNA by using either oligo(dT) or random oligomers of calf thymus DNA as primers. These cDNAs were converted into double-stranded DNA and cloned into pBR322 by standard techniques. The resulting cloned viral DNA fragments were mapped to viral genes by hybridization with Northern blots of intracellular RNA from mouse hepatitis virus strain A59-infected cells. These cDNA clones map in six of the seven viral genes. Clone g344, 1.8 kilobases, is the largest and encompasses gene 5 (which encodes a nonstructural protein) and gene 6 (which encodes the E1 viral glycoprotein) as well as the intergenic regions preceding genes 5, 6, and 7. Sequencing of parts of this cloned DNA show that these three intergenic regions contain a common 11-nucleotide sequence. This sequence shares homology with the 3' end of the viral mRNA leader sequence. Thus, this common intergenic sequence may contain a binding site for a leader RNA that hybridizes to negative-strand viral RNA at the beginning of each gene to prime mRNA synthesis. The different degrees of homology between the leader and its putative binding site may influence the differential rates of transcription of the various viral mRNAs.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 041801814

Download citation: RISBibTeXText

PMID: 2983094


Related references

Nucleotide sequences of the trailer, nucleocapsid protein gene and intergenic regions of Newcastle disease virus strain Beaudette C and completion of the entire genome sequence. Journal of General Virology 79: 2419-2424, 1998

High affinity interaction between nucleocapsid protein and leader/intergenic sequence of mouse hepatitis virus RNA. Journal of General Virology 81(Pt 1): 181-188, 2000

Nucleotide sequence of the genome region encoding the 26S mRNA of eastern equine encephalomyelitis virus and the deduced amino acid sequence of the viral structural proteins. Journal of General Virology 68: 2129-2142, 1987

Nucleotide sequence of the tobacco mosaic virus tomato strain genome and comparison with common strain genome. Journal of Biochemistry 96(6): 1915-1924, 1984

Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome. Journal of Biochemistry 96(6): 1915-1923, 1984

Nucleotide sequence comprising the genome of hepatitis B virus, nucleotide sequence coding the surface antigen of the hepatitis B virus, vectors containing the nucleotide sequences, process for the preparation thereof, and antigen obtained thereby. Official Gazette of the United States Patent & Trademark Office Patents 1237(1), 2000

CDNA nucleotide sequence and primary structure of mouse uterine peptidylarginine deiminase: Detection of a 3'-untranslated nucleotide sequence common in the mRNA of transiently expressed genes and rapid turnover of this enzyme's mRNA in the estrous cycle. European Journal of Biochemistry 215(3): 677-685, 1993

Complete genomic sequence of rapidly replicating strain MB-7 of hepatitis a virus and its characterization in comparison with nucleotide sequence of other hepatitis a virus strain. Molecular Genetics, Microbiology and Virology 25(1): 39-46, 2010

Characteristics of nucleotide substitution in the hepatitis C virus genome: Constraints on sequence change in coding regions at both ends of the genome. Journal of Molecular Evolution 45(3): 238-246, 1997

Nucleotide Sequence of the Tobacco Mosaic Virus (Tomato Strain) Genome and Comparison with the Common Strain Genome1. The Journal of Biochemistry 96(6): 1915-1923, 1984

Nucleotide sequence of the human coronavirus HCV 229E mRNA 4 and mRNA 5 unique regions. Nucleic Acids Research 17(15): 6387, 1989

Nucleotide sequence of the bovine enteric coronavirus BECV F15 mRNA 5 and mRNA 6 unique regions. Nucleic Acids Research 18(5): 1303-1303, 1990

Interactions between the cytoplasmic proteins and the intergenic (promoter) sequence of mouse hepatitis virus RNA: correlation with the amounts of subgenomic mRNA transcribed. Journal of Virology 69(3): 1637-1644, 1995

Elucidation of the genomic nucleotide sequence of bovine coronavirus and analysis of cryptic leader mRNA fusion sites. Advances in Experimental Medicine and Biology 494: 49-55, 2001

In vivo and in vitro transcription of small mRNAs containing a leader sequence from mouse hepatitis virus strain JHM. Journal of Veterinary Medical Science 56(5): 1013-1015, 1994