+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Titration of DnaA protein by oriC DnaA-boxes increases dnaA gene expression in Escherichia coli

Titration of DnaA protein by oriC DnaA-boxes increases dnaA gene expression in Escherichia coli

Embo Journal 6(1): 255-258

Binding of the DnaA protein to its binding sites, the DnaA-boxes (TTATCCACA), was measured by a simple physiological approach. The presence of extra DnaA-boxes in growing cells leads to a derepression of dnaA gene expression, measured as beta-galactosidase activity of a dnaA-lacZ fusion polypeptide. Different DnaA-boxes caused different degrees of derepression indicating that the DnaA protein requires sequences in addition to the DnaA-box for efficient binding. The DnaA-boxes in oriC might act cooperatively in binding of the DnaA protein. The derepressed levels of DnaA protein obtained in a strain carrying an oriC+-pBR322 chimera were very high and sufficient to activate oriC on the chimeric plasmid, which was maintained at a copy number more than three times that of pBR322.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 041815317

Download citation: RISBibTeXText

PMID: 3034578

DOI: 10.1002/j.1460-2075.1987.tb04747.x

Related references

DnaA protein binding to individual DnaA boxes in the Escherichia coli replication origin, oriC. Embo Journal 16(21): 6574-6583, 1997

Multiple pathways regulating DnaA function in Escherichia coli: Distinct roles for DnaA titration by the datA locus and the regulatory inactivation of DnaA. Biochimie 83(1): 13-17, 2001

The presence of two DnaA-binding sequences is required for an efficient interaction of the Escherichia coli DnaA protein with each particular weak DnaA box region. FEMS Microbiology Letters 174(1): 25-31, 1999

Mutational analysis reveals Escherichia coli oriC interacts with both DnaA-ATP and DnaA-ADP during pre-RC assembly. Molecular Microbiology 66(2): 428-439, 2007

Regulation of expression of the dnaA gene in Escherichia coli: role of the two promoters and the DnaA box. New Biologist 2(6): 574-582, 1990

Increased expression of the dnaA gene has no effect on DNA replication in a dnaA+ strain of Escherichia coli. Molecular and General Genetics 192(3): 506-508, 1983

Specific binding of DnaA protein to a DnaA box in the guaB gene of Escherichia coli K12. European Journal of Biochemistry 241(2): 411-416, 1996

Transcriptional repression of the dnaA gene of Escherichia coli by dnaA protein. Molecular and General Genetics 209(3): 518-525, 1987

Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosome. Genes and Development 20(15): 2121-2134, 2006

Transcription termination in the Escherichia coli dnaA gene is not mediated by the internal DnaA box. Journal of Bacteriology 177(7): 1896-1899, 1995

Requirement of the Escherichia coli dnaA gene function for integrative suppression of dnaA mutations by plasmid R 100-1. Molecular and General Genetics 213(1): 163-165, 1988

Occlusion of RNA polymerase by oligomerization of DnaA protein over the dnaA promoter of Escherichia coli. Journal of Biological Chemistry 272(1): 83-88, 1997

Transcription analysis of the dnaA gene and oriC region of the chromosome of Mycobacterium smegmatis and Mycobacterium bovis BCG, and its regulation by the DnaA protein. Microbiology 149(Pt 3): 773-784, 2003

Identification of the region in Escherichia coli DnaA protein required for specific recognition of the DnaA box. Cellular and Molecular Life Sciences 60(9): 1998-2008, 2003

Escherichia coli DnaA protein loads a single DnaB helicase at a DnaA box hairpin. Journal of Biological Chemistry 277(42): 39815-39822, 2002