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Transcription termination and processing of transcripts from tRNA-related Xenopus satellite DNA sequences



Transcription termination and processing of transcripts from tRNA-related Xenopus satellite DNA sequences



European Journal of Biochemistry 164(2): 287-293



Nucleotide sequence homologies have been found between the transcriptional unit of a Xenopus laevis satellite DNA containing 1037-bp monomeric repeats and tRNA-coding genes. Since the tRNA-homologous region forms only part of the transcriptional unit, we have analyzed the size of transcripts being made after injection of cloned DNA into Xenopus oocyte nuclei. Incubation for 6 h mainly results in three RNA species comprising 130, 180 and 196 nucleotides, which originate at the same initiation site but terminate at different T stretches. Longer incubation reveals a complex pattern of smaller RNAs most likely resulting from 3'-end processing of primary transcripts. All these RNAs are related to tRNA, since they contain the tRNA-homologous region which is close to the 5' end of the transcription unit.

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Accession: 041832411

Download citation: RISBibTeXText

PMID: 3569263

DOI: 10.1111/j.1432-1033.1987.tb11056.x


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