Section 42
Chapter 41,840

Translocation of the yeast dolichol-phosphate-mannose synthase into microsomal membranes

Déglon, N.; Krapp, A.; Bron, C.; Fasel, N.

Biochemical and Biophysical Research Communications 174(3): 1337-1342


ISSN/ISBN: 0006-291X
PMID: 1996998
DOI: 10.1016/0006-291x(91)91569-x
Accession: 041839913

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Dolichol-phosphate-mannose synthase catalyzes the formation of Dolichol-phosphate-mannose from Dolichol-phosphate and GDP-mannose. Analysis of the primary amino acid sequence of the yeast enzyme predicts a luminal orientation of the enzyme in the endoplasmic reticulum. We analysed the translocation of the Dolichol-phosphate-mannose synthase into dog pancreatic microsomal membranes: resistance to proteolytic attack provides evidence of its luminal orientation and asks for a reevaluation of the topology of the reaction.

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