Translocation of the yeast dolichol-phosphate-mannose synthase into microsomal membranes
Déglon, N.; Krapp, A.; Bron, C.; Fasel, N.
Biochemical and Biophysical Research Communications 174(3): 1337-1342
ISSN/ISBN: 0006-291X PMID: 1996998 DOI: 10.1016/0006-291x(91)91569-x
Dolichol-phosphate-mannose synthase catalyzes the formation of Dolichol-phosphate-mannose from Dolichol-phosphate and GDP-mannose. Analysis of the primary amino acid sequence of the yeast enzyme predicts a luminal orientation of the enzyme in the endoplasmic reticulum. We analysed the translocation of the Dolichol-phosphate-mannose synthase into dog pancreatic microsomal membranes: resistance to proteolytic attack provides evidence of its luminal orientation and asks for a reevaluation of the topology of the reaction.