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Use of a synthetic oligonucleotide probe to detect strains of non-serovar O1 Vibrio cholerae carrying the gene for heat-stable enterotoxin (NAG-ST)

Hoge, C.W.; Sethabutr, O.; Bodhidatta, L.; Echeverria, P.; Robertson, D.C.; Morris, J.G.

Journal of Clinical Microbiology 28(6): 1473-1476

1990

ISSN/ISBN: 0095-1137
PMID: 2380369
Accession: 041914323
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A synthetic oligonucleotide probe was developed to identify the gene for the heat-stable enterotoxin (NAG-ST) of non-serovar O1 Vibrio cholerae. Of 103 non-O1 V. cholerae isolates from Thailand, 31 isolates from Mexico, and 47 isolates from patients in the United States, only 7 (all from Thailand) hybridized with the probe. Probe-positive strains produced significantly higher fluid accumulations in infant mice than probe-negative strains.

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