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VIP receptors and control of short circuit current in the human intestinal clonal cell line Cl.19A

Rouyer-Fessard, C.; Augeron, C.; Grasset, E.; Maoret, J.J.; Laboisse, C.L.; Laburthe, M.

Experientia 45(11-12): 1102-1105

1989

ISSN/ISBN: 0014-4754
PMID: 2557231
DOI: 10.1007/bf01950169
Accession: 041932001
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At the maximally effective concentration of 10 nM, VIP induced a marked (12.5-fold stimulation above basal), and sustained increase in short circuit current in the human intestinal epithelial cell line Cl.19A grown on permeable filters and placed in Ussing chambers. Half-maximal increase of Isc was observed for 0.1 nM VIP. This was well correlated with the VIP-stimulated adenylate cyclase activity (ED50:0.07 nM). Binding studies using 125I-VIP indicated that Cl.19A cells express a peptide-specific VIP receptor with a dissociation constant of 0.07 nM. Covalent labeling of receptors followed by SDS-PAGE analysis of membrane proteins resulted in the identification of a 63,000 dalton binding protein in Cl.19A cells.

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