+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Vaccination against lethal coronavirus-induced encephalitis with a synthetic decapeptide homologous to a domain in the predicted peplomer stalk



Vaccination against lethal coronavirus-induced encephalitis with a synthetic decapeptide homologous to a domain in the predicted peplomer stalk



Journal of Virology 62(8): 3032-3036



A surface probability method was used to select a decapeptide homologous to residues 993 to 1002 of the peplomer protein E2 of murine hepatitis virus strain JHM, a neurotropic coronavirus. This sequence of amino acids corresponded to a minor peak on a hydrophilicity plot. Immunization of mice with the chemically synthesized peptide coupled to keyhole limpet hemocyanin elicited high levels of neutralizing antibody and protected against lethal virus challenge. Protection correlated with a critical level of antipeptide antibody, which could be reached after a single inoculation. These results suggest that an appropriate antibody response to a highly restricted, surface-exposed domain of this viral protein is critical in determining the outcome of infection of the central nervous system. This sequence is located in the C-terminal fifth of the E2 peplomers, between two predicted coiled-coil structures.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 041932303

Download citation: RISBibTeXText

PMID: 2455823


Related references

The predicted primary structure of the peplomer protein E2 of the porcine coronavirus transmissible gastroenteritis virus. Journal of General Virology 68: 1883-1890, 1987

Vaccination with synthetic decapeptide epitope of human sperm FA-1 causes contraception in female rabbits. EOS-Rivista di Immunologia ed Immunofarmacologia 12(2): 122, 1992

Microglia are required for protection against lethal coronavirus encephalitis in mice. Journal of Clinical Investigation 128(3): 931-943, 2018

Conformational change of the coronavirus peplomer glycoprotein at pH 8.0 and 37 degrees C correlates with virus aggregation and virus-induced cell fusion. Journal of Virology 64(6): 3042-3050, 1990

'Wave-type' structure of a synthetic hexaglycosylated decapeptide: A part of the extracellular domain of human glycophorin A. Journal of Biomolecular NMR 14(1): 33-45, 1999

Sequence and structure of the coronavirus peplomer protein. Advances in Experimental Medicine and Biology 218: 31-38, 1987

A predicted receptor-binding and critical neutralizing domain in S protein of the novel human coronavirus HCoV-EMC. Journal of Infection 66(5): 464-466, 2013

Prediction of quaternary assembly of SARS coronavirus peplomer. Biochemical and Biophysical Research Communications 325(4): 1210-1214, 2004

Expression of the peplomer glycoprotein of murine coronavirus JHM using a baculovirus vector. Virology 173(2): 615-623, 1989

Functional differences in the peplomer glycoproteins of feline coronavirus isolates. Journal of Virology 61(8): 2655-2657, 1987

Bovine coronavirus peplomer glycoproteins: detailed antigenic analyses of S1, S2 and HE. Journal of General Virology 73: 1725-1737, 1992

The peplomer protein sequence of the M41 strain of coronavirus IBV and its comparison with Beaudette strains. Virus Research 5(2-3): 253-263, 1986

Hybridoma antibodies to the murine coronavirus JHM: characterization of epitopes on the peplomer protein (E2). Journal of General Virology 65: 1931-1942, 1984

Characterization in vitro of an autocatalytic processing activity associated with the predicted 3C-like proteinase domain of the coronavirus avian infectious bronchitis virus. Journal of Virology 70(3): 1923-1930, 1996

Peptides corresponding to the predicted heptad repeat 2 domain of the feline coronavirus spike protein are potent inhibitors of viral infection. Plos one 8(12): E82081, 2013