+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Viral transcription in human keratinocyte cell lines immortalized by human papillomavirus type-16



Viral transcription in human keratinocyte cell lines immortalized by human papillomavirus type-16



Virology 183(1): 331-342



Human papillomavirus type-16 (HPV-16) transcription in two human keratinocyte cell lines (HPK) immortalized by transfection of viral DNA in vitro was analyzed by nucleotide sequencing of cDNA clones, and in addition by primer extension analysis and S1 nuclease and exonuclease VII digestion of poly(A)+ RNA. A novel mRNA species which probably initiates in the E7 ORF and in which the 5'-part of the E1 ORF (splice donor at position (pos.) 880) is joined to an exon comprising the entire E2 ORF (splice acceptor at pos. 2708) was found in both cell lines. This mRNA has the potential to encode a full-length E2 protein, which is known to function as a repressor of transcription initiated at P97. cDNAs derived from the late region of the viral genome and the use of a late polyadenylation signal at pos. 7320-7325 are described. In agreement with RNA data published by others the major promoter for HPV-16 transcription is located at pos. 97. mRNA species encoding full-length or truncated forms of the E6 protein, and species characterized by an E1i [symbol see text] E4 splice junction (which provided the E4 open reading frame (ORF) with an ATG triplet) were identified.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 041956851

Download citation: RISBibTeXText

PMID: 1647072

DOI: 10.1016/0042-6822(91)90146-3


Related references

Viral integration and fragile sites in human papillomavirus-immortalized human keratinocyte cell lines. Genes, Chromosomes & Cancer 5(2): 150-157, 1992

Chromosomal organization of viral integration sites in human papillomavirus-immortalized human keratinocyte cell lines. Cancer Genetics & Cytogenetics. 72(1): 39-43, 1994

Increased sensitivity of human papillomavirus type 16 immortalized human keratinocyte lines to growth control by retinoic acid. Proceedings of the American Association for Cancer Research Annual Meeting 31: 327, 1990

Cytogenetic analysis of eight human papillomavirus immortalized human keratinocyte cell lines. International Journal of Cancer 44(6): 1124-1131, 1989

Infectious human papillomavirus type 31b: purification and infection of an immortalized human keratinocyte cell line. Journal of General Virology 83(Pt 11): 2753-2763, 2002

Establishment of the human papillomavirus type 16 (HPV-16) life cycle in an immortalized human foreskin keratinocyte cell line. Virology 262(2): 344-354, 1999

Viral integration and fragile sites in human papillomavirus-lmmortalized human keratinocyte cell lines. Genes, Chromosomes and Cancer 5(2): 150-157, 1992

Integration of human papillomavirus 16 DNA and genomic rearrangements in immortalized human keratinocyte lines. Cancer Research 50(4): 1316-1323, 1990

Expression of prostatic factors measured by reverse transcription polymerase chain reaction in human papillomavirus type 18 deoxyribonucleic acid immortalized prostate cell lines. Urology. 51(4): 657-662, Il, 1998

Differentiation-induced and constitutive transcription of human papillomavirus type 31b in cell lines containing viral episomes. Journal of Virology 66(10): 6070-6080, 1992

Use of amphotropic retroviruses expressing human papillomavirus 16 E6 and E7 to determine the consequences of acute expression of the viral oncogenes and to establish immortalized human cell lines. Radiation Oncology Investigations 3(6): 315-319, 1995

Viral integration sites in human papilloma virus-33-immortalized cervical keratinocyte cell lines. Cancer Genetics & Cytogenetics 90(1): 63-69, 1996

Leukoregulin and gamma-interferon inhibit human papillomavirus type 16 gene transcription in human papillomavirus-immortalized human cervical cells. Cancer Research 52(2): 456-463, 1992

Elevated wild-type p53 protein levels in human epithelial cell lines immortalized by the human papillomavirus type 16 E7 gene. Virology 198(1): 169-174, 1994

Distinct squamous phenotypes of human ectocervical cell lines immortalized by human papillomavirus type 16. Molecular & Cellular Differentiation 2(2): 141-157, 1994