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cDNA sequence of two distinct pituitary proteins homologous to Kex2 and furin gene products: tissue-specific mRNAs encoding candidates for pro-hormone processing proteinases



cDNA sequence of two distinct pituitary proteins homologous to Kex2 and furin gene products: tissue-specific mRNAs encoding candidates for pro-hormone processing proteinases



Dna and Cell Biology 9(10): 789-789



Based on the concept of sequence conservation around the active sites of serine proteinases, the polymerase chain reaction was used to amplify mRNA and enabled a 260-bp probe to be generated which was used to screen a mouse pituitary cDNA library. The primers used were derived from the cDNA sequence of active sites Ser and Asn of human furin. Two cDNA sequences were obtained from a number of positive clones. These code for 2 similar but distinct structures (mPC1 and mPC2), each being homologous to yeast Kex2 and human furin. In situ hybridization and northern blots demonstrated tissue and cellular specificity of expression, only within endocrine and neuroendocrine cells. These data suggest that mPC1 and mPC2 represent prime candidates for tissue-specific pro-hormone converting proteinases.

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Accession: 041997279

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PMID: 2264933

DOI: 10.1089/dna.1990.9.789


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