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virG of Agrobacterium tumefaciens plasmid pTiC58 encodes a DNA-binding protein



virG of Agrobacterium tumefaciens plasmid pTiC58 encodes a DNA-binding protein



Molecular Microbiology 3(3): 411-419



Virulence genes of the Agrobacterium tumefaciens Ti plasmid are positively regulated by the products of virA and virG. To study the DNA-binding properties of the VirG protein, a translational fusion between virG and the trpE gene of Escherichia coli was constructed, and antiserum was raised against the encoded fusion protein. Using this antiserum, a protein of Mr congruent to 29,000, a size similar to that calculated from the virG nucleotide sequence, was detected in an E. coli strain harbouring a virG expression vector. Both the virG protein and the fusion protein were found, by filter-binding and gel retardation analyses, to bind DNA nonspecifically. These data support an existing model for the two-component regulatory systems of bacteria.

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Accession: 041999888

Download citation: RISBibTeXText

PMID: 2664419

DOI: 10.1111/j.1365-2958.1989.tb00186.x



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