3H-thymidine paper strip modification of the lymphocyte transformation test. III. Whole blood lymphocyte mixed culture using a cryopreserved stimulating cell mixture
Schütt, C.; Wegener, S.; Knippel, E.
Acta Biologica et Medica Germanica 39(10): 1045-1050
1980
ISSN/ISBN: 0001-5318 PMID: 6452781 Accession: 042020821
In comparison to the classical mixed lymphocyte culture (MLC) method a MLC technique using whole blood cultures of responding cells and a pool of prepared stimulating cells from 10 healthy blood donors is described. The reproducibility of cryopreservation of stimulating lymphocytes was proven by means of cell electrophoresis.
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