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A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes

A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes

Gene 10(3): 249-259

ISSN/ISBN: 0378-1119

PMID: 6254843

DOI: 10.1016/0378-1119(80)90054-2

Lambda derivatives are described that can be used for cloning DNA fragments of about 20 kilobase pairs (kb) generated by restriction enzymes EcoRi, HindIII, BamHI, MboI and BglII. Recombinants can be selected by their Spi- phenotype and their propagation is facilitated by the presence of a chi site.

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Accession: 042029181

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