A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes

Loenen, W.A.; Brammar, W.J.

Gene 10(3): 249-259

1980


ISSN/ISBN: 0378-1119
PMID: 6254843
DOI: 10.1016/0378-1119(80)90054-2
Accession: 042029181

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 0-6 h
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
Lambda derivatives are described that can be used for cloning DNA fragments of about 20 kilobase pairs (kb) generated by restriction enzymes EcoRi, HindIII, BamHI, MboI and BglII. Recombinants can be selected by their Spi- phenotype and their propagation is facilitated by the presence of a chi site.