+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A microcytotoxicity assay for thyroid-specific cytotoxic antibody, antibody-dependent cell-mediated cytotoxicity and direct lymphocyte cytotoxicity using human thyroid cells



A microcytotoxicity assay for thyroid-specific cytotoxic antibody, antibody-dependent cell-mediated cytotoxicity and direct lymphocyte cytotoxicity using human thyroid cells



Journal of Immunological Methods 48(2): 241-250



A microcytotoxicity assay for detection of thyroid-specific complement-dependent cytotoxic antibody (cytotoxic antibody), antibody-dependent cell-mediated cytotoxicity (ADCC) and direct lymphocyte cytotoxicity was developed using human, thyroid epithelial cells as targets. Thyroid tissue was obtained from patient with Graves' disease and was treated with collagenase and then trypsin. The red blood cells, interfollicular fibroblasts and infiltrating lymphoid cells in thyroid tissue from patients with Graves' disease could be removed by this procedure. To obtain entirely single cells as target cells, the suspension of dispersed thyroid epithelial cells was allowed to stand for 1 h at 4 degree C in culture medium to allow cell clumps to settle. For assay of cytotoxic antibody, a mixture of the single target cells, patient's serum and human complement was incubated in microwells for 18 h. After removing the detached cells, remaining target cells in the wells were fixed, stained and counted to assess cytotoxicity. For assay of ADCC and of direct lymphocyte cytotoxicity, target thyroid cells were precultured in the microwells for 18 h. Then effector cells with or without patient's serum were added and cultured further for 24-72 h. Cytotoxicity was assessed as described above. Adherence of effector cells to target thyroid cells sometimes disturbed the enumeration of target cells when the effector/target cell ratio was high. With this microassay system 3 different cytotoxic immune reactions against human thyroid cells could be measured quantitatively at the same time on 2-3 ml blood samples.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 042077253

Download citation: RISBibTeXText

PMID: 6895761

DOI: 10.1016/0022-1759(82)90198-3


Related references

Antibody dependent cell mediated cytotoxicity toward human o positive red cells coated with anti d antibody comparison between lymphocyte and monocyte antibody dependent cell mediated cytotoxicity activity. Acta Medica Okayama 39(2): 77-90, 1985

Modulation of cytotoxic T lymphocyte, natural killer cell, antibody-dependent cellular cytotoxicity, and antibody-dependent complement-mediated cytotoxicity by Vernonia cinerea L. and vernolide-A in BALB/c mice via enhanced production of cytokines IL-2 and IFN-γ. Immunopharmacology and Immunotoxicology 34(1): 46-55, 2012

Spontaneous lymphocyte-mediated cytotoxicity and antibody-dependent cell cytotoxicity of human effector cells. Folia Haematologica 110(6): 831-842, 1983

Human lymphocyte-dependent antibody mediated cytotoxicity and direct lymphocyte cytotoxicity against non-HL-A antigens. Nature 249(454): 263-265, 1974

Direct lymphocytotoxicity and antibody-dependent cell-mediated cytotoxicity in thyroid diseases. La Revue de Medecine Interne 1(1): 39-44, 1980

Antibody-dependent cell mediated cytotoxicity against human thyroid cells in Hashimoto's thyroiditis but not Graves' disease. Journal of Clinical Endocrinology and Metabolism 59(4): 734-738, 1984

Anti-Sp17 monoclonal antibody with antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity activities against human ovarian cancer cells. Medical Oncology 29(4): 2923-2931, 2012

Antibody-dependent cell-mediated cytotoxicity against orbital target cells in thyroid-associated ophthalmopathy and related disorders; close relationship between serum cytotoxic antibodies and parameters of eye muscle dysfunction. Journal of Endocrinological Investigation 19(6): 334-341, 1996

Antibody inducing lymphocyte cytotoxicity (ADCC) and lymphocyte-mediated cytotoxicity in auto-immune thyroid diseases. Pathologie-Biologie 29(4): 211-215, 1981

Human natural cell-mediated cytotoxicity against fetal fibroblasts. IV. Comparison of cytotoxic activity with antibody-dependent cell-mediated cytotoxicity. Scandinavian Journal of Immunology 9(3): 239-245, 1979

Cytotoxicity mediated by human lymphocyte-dependent antibody in a rapid assay with adherent target cells. Journal of Immunology 110(6): 1672-1681, 1973

Varicella zoster virus vzv specific cytotoxicity antibody dependent cell mediated cytotoxicity adcc natural killer nk cell activity and lymphocyte proliferation lp responses in the elderly. Clinical Research 37(4): 909A, 1989

Inhibition of human lymphocyte natural cytotoxicity and antibody-dependent cell-mediated cytotoxicity by K-76 COONa, a reagent that blocks complement activity. Journal of Immunology 133(1): 408-414, 1984

Antibody-dependent cell-mediated cytotoxicity (ADCC) assay for specific IgG antibody produced in vitro. Journal of Immunological Methods 36(3-4): 243-254, 1980

Simultaneous expression of cell-mediated cytotoxicity and antibody-dependent cellular cytotoxicity to a syngeneic rat lymphoma: separation and partial characterization of two types of cytotoxic cells. Cellular Immunology 14(2): 193-205, 1974