+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

A morphometric study of vascular smooth muscle cells in culture



A morphometric study of vascular smooth muscle cells in culture



In Vitro 16(4): 337-345



Cultured arterial smooth muscle cells derived from different times in culture, different passages, and different species were evaluated by a combination of transmission electron microscopy and morphometry. The morphometric studies focused on point counting and monitored the following cellular components: lysosomes, myofilaments, mitochondria, ribosomes, and rough endoplasmic reticulum (RER). Percent volume composition values for the organelles involved in protein synthesis, namely ribosomes and RER, show significant fluctuations with time. Consistent with these observations, the cells showed increasing myofilaments during the early weeks in culture, which subsequently decreased significantly. The data also indicate that rabbit cells in culture may become synthetically quiescent with time and the distribution of cellular components is altered with each succeeding passage. Cultured calf (bovine) cells exhibit similar activity periods compared to rabbit but show a significantly higher lysosomal and lower myofilament content than rabbit. Calf cells could not be maintained for longer than 21 days in the absence of ascorbate, whereas ascorbate affects the ultrastructure of rabbit cells less dramatically. Age, passage, and donor, among others, are important considerations for studying in vitro smooth muscle cells. With proper morphologic and morphometric monitoring, these smooth muscle cell culture systems can be important tools in the study of aging or pathologic processes, or both.

(PDF emailed within 0-6 h: $19.90)

Accession: 042080647

Download citation: RISBibTeXText

PMID: 7190547

DOI: 10.1007/bf02618340


Related references

A Morphometric Study of Vascular Smooth Muscle Cells in Culture. In Vitro 16(4): 337-345, 1980

Inhibitory role of reactive oxygen species in the differentiation of multipotent vascular stem cells into vascular smooth muscle cells in rats: a novel aspect of traditional culture of rat aortic smooth muscle cells. Cell and Tissue Research 362(1): 97-113, 2016

Supernatant of vascular smooth muscle cell and syngeneic splenocyte co culture inhibits the proliferation of smooth muscle cells. FASEB Journal 6(4): A1043, 1992

Co culture of vascular endothelium and smooth muscle cells effects of heparin on smooth muscle proliferation. Journal of Cell Biology 97(5 PART 2): 87A, 1983

Characterization of vascular smooth muscle progenitor cells in circulating human peripheral blood Potential origin of intimal smooth muscle cells in vascular lesions and involvement in vascular inflammation. Circulation 108(17 Suppl.): IV-285, October 28, 2003

Morphometric analysis of vascular smooth muscle cells by scanning electron microscopy. International Review of Experimental Pathology 36: 31-52, 1996

Effect of lyso-phosphatidylcholine and Schnurri-3 on osteogenic transdifferentiation of vascular smooth muscle cells to calcifying vascular cells in 3D culture. Biochimica et Biophysica Acta 1830(6): 3828-3834, 2013

Effects of co-culture with vascular endothelial cells on the renin-like enzyme production in vascular smooth muscle cells. Clinical and Experimental Hypertension. Part A, Theory and Practice 11(3): 447-457, 1989

Co-culture of endothelial cells and smooth muscle cells in a flow environment: An improved culture model of the vascular wall?. Cells and Materials 5(2): 115-124, 1995

Changes in sodium pump expression during proliferation of canine vascular smooth smooth muscle cells vsmc in primary culture. Journal of Cellular Biochemistry Suppl. (13 PART E): 188, 1989

Indirect co‑culture of vascular smooth muscle cells with bone marrow mesenchymal stem cells inhibits vascular calcification and downregulates the Wnt signaling pathways. Molecular Medicine Reports 13(6): 5141-5148, 2017

Application of computerized image analyzing system in morphometric study of vascular smooth muscle cells. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 14(4): 346-8, 372, 2001

DFMG reverses proliferation and migration of vascular smooth muscle cells induced by co-culture with injured vascular endothelial cells via suppression of the TLR4-mediated signaling pathway. Molecular Medicine Reports 17(4): 5692-5699, 2018

Vascular smooth muscle cells on hyaluronic acid: culture and mechanical characterization of an engineered vascular construct. Tissue Engineering 10(5-6): 699-710, 2004

A novel smooth muscle-specific enhancer regulates transcription of the smooth muscle myosin heavy chain gene in vascular smooth muscle cells. Journal of Biological Chemistry 270(52): 30949-30957, 1995