A second form of energy transfer factor B and a new factor (factor C) of mitochondrial oxidative phosphorylation
Lam, K.W.; Karunakaran, M.E.; Sanadi, D.R.
Biochemical and Biophysical Research Communications 39(3): 437-443
ISSN/ISBN: 0006-291X PMID: 4316207 DOI: 10.1016/0006-291x(70)90597-8
The isolation and characterization of Factor B, a mitochondrial protein involved in oxidative phosphorylation, has been described previously. The occurrence of another form of the factor, designated Factor B′, which can be separated from Factor B by CM-cellulose chromatography, has been established. Like Factor B, it is inhibited by p-chloromercuriphenylsulfonate and an antiserum to Factor B. The preparation contains, in addition, another energy transfer factor, Factor C, which stimulates ATP-driven Nad reduction by succinate in a highly depleted particle beyond the stimulation produced by Factor B.