Activation of brain phenylethanolamine-N-methyltransferase by triton-X-100: time related differences in the enzyme activation

Chaudhuri, S.; Parvez, H.; Parvez, S.

Biochemical and Biophysical Research Communications 94(2): 522-528

1980


ISSN/ISBN: 0006-291X
PMID: 7396915
DOI: 10.1016/0006-291x(80)91262-0
Accession: 042165408

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Abstract
The effect of different concentrations of Triton-X-100 (0.2 to 5 %) on the activity of enzyme phenylethanolamine-N-methyltransferase (PNMT) in the brain and adrenal was studied. The addition of 0.2 % Triton-X-100 to the 0.9 % KCl homogenization media resulted in 180 % activation of the brain PNMT. The similar content of this detergent added to the adrenal Pnmt preparation had no marked effect on enzyme activity. Rising Triton-X-100 concentrations from 0.2 % to 5 % resulted in higher activation of brain Pnmt activity but the adrenal enzyme remained rather stable. An exposure of 15 minutes of brain Pnmt preparation to Triton-X-100 was the optimal interval to evoke the maximal increase in enzyme activity. This activation of brain Pnmt by Triton-X-100 was observable up to 24 hours after the addition of the detergent.