EurekaMag
+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Alkaline phosphatase. Possible induction by cyclic AMP after cholera enterotoxin administration


Alkaline phosphatase. Possible induction by cyclic AMP after cholera enterotoxin administration



Journal of Clinical Investigation 52(11): 2928-2934



ISSN/ISBN: 0021-9738

PMID: 4356003

The present studies were undertaken to determine the role, if any, of cyclic 3',5'-adenosine monophosphate (cyclic AMP) as a chemical inducer of rat liver alkaline phosphatase. Cholera enterotoxin, given intravenously to rats, led to a rapid rise in the activity of hepatic adenyl cyclase that was 7(1/2) times greater than control values in 6 h. Cyclic AMP levels were also significantly increased above control values while the activity of cyclic nucleotide phosphodiesterase was unchanged. Hepatic alkaline phosphatase activity was increased 5(1/2) times above control in 12 h, but its rise followed that of adenyl cyclase and cyclic AMP by several hours. Cycloheximide inhibited the rise of hepatic alkaline phosphatase but not that of adenyl cyclase. The administration of glucagon, a known stimulator of hepatic adenyl cyclase, and of dibutyryl cyclic AMP, led to similar striking increases in hepatic alkaline phosphatase activity. This alkaline phosphatase increase was blocked by the prior administration of cycloheximide. Bile duct ligation, a known stimulator of hepatic alkaline phosphatase activity, failed to produce any significant changes in adenyl cyclase or cyclic AMP. Concomitant treatment of rats with bile duct ligation and cholera enterotoxin or bile duct ligation and glucagon, had no additive effect on the increase in hepatic alkaline phosphatase activity, although the increase occurred earlier. These results suggest that: (a) cyclic AMP may act as an inducer of hepatic alkaline phosphatase: (b) the stimulation of hepatic alkaline phosphatase by cholera enterotoxin is mediated by cyclic AMP; (c) the rise in hepatic alkaline phosphatase following bile duct ligation is not mediated by cyclic AMP; (d) the same alkaline phosphatase in rat liver may be induced by two (or more) mechanisms, only one of which requires cyclic AMP.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 042210838

Download citation: RISBibTeXText

Related references

Effects of cholera enterotoxin, glucagon, and dibutyryl cyclic AMP on rat liver alkaline phosphatase, bile flow, and bile composition. Gastroenterology 70(4): 577-581, 1976

Induction of alkaline phosphatase activity by n 6 mono butyryl cyclic amp specific cyclic amp effect on gene transcription. Clinical Research 28(2): 517A, 1980

Hepatic alkaline phosphatase possible induction by cyclic amp. Gastroenterology 62(1): 171, 1972

The cyclic AMP-mediated induction of alkaline phosphatase in mouse L-cells. Journal of Biological Chemistry 256(3): 1396-1403, 1981

Cyclic amp mediated cortisol induction of hela cell alkaline phosphatase. Huijing, F.y.c. Lee (ed.). Miami Winter Symposia, Vol. 5. Protein Phosphorylation In Control Mechanisms. Miami, Fla., U.s.a. 15-16,: New York, N.y., U.s.a.; London, England. 301, 1973

Stimulation of phosphorylation of rat brush-border membrane proteins by Escherichia coli heat-stable enterotoxin, cholera enterotoxin and cyclic nucleotides, and its inhibition by protein kinase inhibitors, isoquinolinesulfonamides. Microbial Pathogenesis 8(6): 421-431, 1990

Stimulation of phosphorylation of rat brush-border membrane proteins by Escherichia coli heat-stable enterotoxin, cholera enterotoxin and cyclic nucleotides, and its inhibition by protein kinase inhibitors, isoquinolinesulfonamides. Microbial Pathogenesis 8(6): 421-431, 1990

Induction of germ-cell alkaline phosphatase by butyrate and cyclic AMP in BeWo choriocarcinoma cells. Biochemical Journal 296: 59-65, 1993

Increase of cyclic amp levels in cultured hela 65 cells during induction of alkaline phosphatase by prednisolone. In Vitro 8(5): 439-440, 1973

The role of glycosylation in the cyclic amp mediated induction of plasma membrane alkaline phosphatase in mouse l cells. Federation Proceedings 38(3 Part 1): 791, 1979

Induction of osteoblastic cell differentiation by forskolin. Stimulation of cyclic AMP production and alkaline phosphatase activity. Biochimica et Biophysica Acta 838(1): 49-53, 1985

Induction of alkaline phosphatase activity by dibutyryl adenosine 3':5'-cyclic monophosphate in aneuploid rat liver cells. Experimental Cell Research 87(1): 8-14, 1974

Induction of alkaline phosphatase in mouse l cells by overexpression of the catalytic subunit of cyclic amp dependent protein kinase. Journal of Biological Chemistry 265(22): 13181-13189, 1990

Induction of alkaline phosphatase by cyclic AMP or its dibutyryl derivative in a hybrid line between mouse and Chinese hamster in culture. Biochemical and Biophysical Research Communications 46(1): 305-311, 1972

Induction of alkaline phosphatase in choriocarcinoma cells by 1-beta-D-arabinofuranosyl-cytosine, mitomycin C, phleomycin, and cyclic nucleotides. Journal of Cellular Physiology 92(2): 221-231, 1977