+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

An evaluation of the homogeneous substrate-labeled fluorescent immunoassay for tobramycin in serum



An evaluation of the homogeneous substrate-labeled fluorescent immunoassay for tobramycin in serum



Methods and Findings in Experimental and Clinical Pharmacology 4(4): 249-253



The homogeneous substrate-labeled fluorescent immunoassay for tobramycin in serum was evaluated. Standard curves are linear and reproducible. The within-run coefficient of variation for control samples containing low, medium and high concentrations of tobramycin ranged from 1.9 to 6.5%; the between-run coefficient of variation was 11% for a control sample at low concentration. The accuracy measured on spiked samples was good and comparable to the accuracy measured by a microbiological assay; it was somewhat better than for the radioimmunoassay. For patient samples, the results with this method correlated well (r - 0.914) with results obtained by radioimmunoassay.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 042242120

Download citation: RISBibTeXText

PMID: 6750285


Related references

Homogeneous substrate labeled fluorescent immunoassay for determining tobramycin concentrations in human serum. Pal S B (Ed ) Enzyme Labelled Immunoassay Of Hormones And Drugs Proceedings Of The International Symposium Ulm, West Germany, July 10-11, Xxv+475p Illus Walter De Gruyter: Berlin, West Germany; New York, N Y , Usa ISBN 3-11-007539-3: 387-403, 1978

Homogeneous substrate-labeled fluorescent immunoassay for IgG in human serum. Journal of Immunological Methods 42(1): 93, 1981

Homogeneous substrate-labeled fluorescent immunoassay for theophylline in serum. Clinical Chemistry 27(1): 22-26, 1981

Homogeneous substrate labeled fluorescent immunoassay for ethosuximide in human serum. Clinical Chemistry 28(7): 1664, 1982

Homogeneous substrate-labeled fluorescent immunoassay for human serum albumin. Journal of Immunoassay 4(2): 159-174, 1983

Homogeneous substrate labeled fluorescent immunoassay for immuno globulin g in human serum. Journal of Immunological Methods 42(1): 93-104, 1981

Automation of the homogeneous substrate-labeled fluorescent immunoassay for theophylline in serum: application to the determination of phenytoin and phenobarbital in serum. Therapeutic Drug Monitoring 4(4): 419-425, 1982

Homogeneous substrate-labeled fluorescent immunoassay for disopyramide in human serum: semi- and fully automated procedures. Clinical Chemistry 32(2): 378-381, 1986

The homogeneous substrate-labeled fluorescent immunoassay. Methods in Enzymology 74 Pt C: 79-87, 1981

Homogeneous substrate-labeled fluorescent immunoassay for carbamazepine. Epilepsia 23(4): 391-398, 1982

Evaluation of a substrate labeled fluorescent immunoassay for the determination of quinidine in serum. Clinical Biochemistry 15(2): 98, 1982

Determination of phenytoin in serum by nonisotopic immunoassays enzyme multiplied immunoassay technique fluorescence polarization immunoassay nephelometric inhibition immunoassay substrate labeled fluorescent immunoassay and gas chromatography. Journal of Clinical Chemistry & Clinical Biochemistry 20(9): 665-666, 1982

A solid phase homogeneous substrate labeled fluorescent immunoassay for theophylline comparison of fixed time and kinetic data analysis. Clinical Chemistry: 1666, 1982

Fully automated system approach to homogeneous nonisotopic immunoassays 2. total automation of the substrate labeled fluorescent immunoassay for therapeutic drugs. Clinical Chemistry 28(7): 1674, 1982