+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Antigen-induced histamine release from guinea pig basophils



Antigen-induced histamine release from guinea pig basophils



International Archives of Allergy and Applied Immunology 64(3): 241-248



Guinea pig blood was found to contain an average of 106 +/- 21 ng/ml of histamine. Of this total, approximately 85-90% was of platelet origin and the rest from basophils. Basophils contain about 0.72 pg of histamine/cell. Concanavalin A (1-5 microgram/ml) induced the release of approximately 65% of the basophilic histamine. When basophils were isolated from animals sensitized to ovalbumin or keyhole limpet hemocyanin, addition of the appropriate antigen induced histamine release at concentrations of 0.01 microgram/ml or lower. Individual animals were studied over time by repetitive bleeding. The circulating basophils remained sensitized for at least 17 weeks postsensitization. However, release did not occur if animals had been sensitized less than 7 days earlier. This assay facilitates the investigation of basophil sensitization since animals can be studied on several occasions following immunization. The mechanisms, timing and role of basophil sensitization in various types of immune and hypersensitivity reactions can now be evaluated.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 042293586

Download citation: RISBibTeXText

PMID: 6163732

DOI: 10.1159/000232700


Related references

Further studies on the process of histamine release from sensitized guinea-pig bone marrow basophils by antigen. International Archives of Allergy and Applied Immunology 37(4): 376-384, 1970

Hiv antigen induced release of histamine from basophils from hiv infected patients. Allergy 46(3): 206-212, 1991

De sensitization of rabbit basophils for antigen induced histamine release. Federation Proceedings 38(3 Part 1): 1019, 1979

H2 antihistamines augment antigen-induced histamine release from human basophils in vitro. Journal of Immunology 129(5): 2113-2115, 1982

IgE-mediated histamine release from human basophils: differences between antigen E- and anti-IgE-induced secretion. International Archives of Allergy and Applied Immunology 65(3): 339-348, 1981

The flavonoids: a brief review and study of effects on antigen-induced histamine release from human basophils. Transactions of the American Clinical and Climatological Association 92: 234-252, 1980

Sensitization of circulating basophils in guinea pig recipients of passive transfer of cutaneous basophil hypersensitivity (CBH) with immune serum: antigen-specific histamine release in vitro. Journal of Immunology 130(5): 2104-2108, 1983

Antigen-induced histamine release from mast cells and basophils after hydrocortisone and disodium cromoglycate treatment. Agents and Actions 11(1-2): 107-109, 1981

Effects of flavonoids and transitional metal cations on antigen-induced histamine release from human basophils. Biochemical Pharmacology 31(7): 1449-1453, 1982

Effects of arachidonic acid and its metabolites on antigen induced histamine release from human basophils in vitro. Journal of Immunology 123(4): 1669-1677, 1979

Quantification of ultrastructural variations in enriched blood basophils: correlation of morphological changes and antigen-induced histamine release. Immunology 47(1): 41-47, 1982

HIV antigen-induced release of histamine from basophils from HIV infected patients. Mechanism and relation to disease progression and immunodeficiency. Allergy 46(3): 206-212, 1991

Differential inhibition of histamine release from human basophils induced by antigen, anti-IgE or N-formyl-L-methionyl-L-leucyl-L-phenylalanine. International Archives of Allergy and Applied Immunology 77(1-2): 222-224, 1985

The effect of short-term immunotherapy with Pollinex on anti-IgE and antigen-induced histamine release from sensitive human basophils. Archivum Immunologiae et Therapiae Experimentalis 33(6): 777-782, 1985

Differential inhibition of histamine release from human basophils induced by antigen anti immunoglobulin e or n formyl l methionyl l leucyl l phenylalanine. International Archives of Allergy and Applied Immunology 77(1-2): 222-224, 1985