+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Antigens and allergens in Dermatophagoïdes farinae mite. I. Immunochemical and physicochemical study of two allergenic fractions from a partially-purified Dermatophagoïdes farinae mite extract



Antigens and allergens in Dermatophagoïdes farinae mite. I. Immunochemical and physicochemical study of two allergenic fractions from a partially-purified Dermatophagoïdes farinae mite extract



Immunology 44(2): 239-247



The fractionation of a partially-purified extract of Dermatophagoïdes farinae mite culture has been undertaken by gel filtration. Two fractions were isolated. One, P25, is a protein-rich fraction with mol. wt about 25,000. The other, GP8, is a polysaccharide-rich fraction with mol. wt around 8,000. By crossed immunoelectrophoresis, we detected eleven antigens in the partially-purified dialysed D. farinae extract (Df 80d) as well as in P25 fraction. One of them, ag 11, seems the most important allergen since in crossed-radio immunoelectrophoresis experiments it displays the faster radiostaining, implying that it binds the greatest part of the mite-specific IgE present in a pool of sera from mite-sensitive patients. By crossed-line immunoelectrophoresis, we demonstrated the absence of ag 11 in GP8, in which only ag 5 and ag 6 were identifiable. By radioalloergosorbent tests (RAST), it was found that P25- and GP8- coated paper discs can fix specific IgE induced in the majority of D. farinae sensitive patients. Defining a 'major allergen' as an allergen to which the majority of sensitive patients develop specific IgE, both P25 and GP8 do appear to contain at least one major allergen. By RAST inhibition method, using Df 80d as a solid phase, the allergenic activity of P25 appeared as slightly higher than that of Df 80d, whereas GP8 displayed a very weak inhibitor capacity. Thus, the allergic specificity of GP8 differs from that of Df 80d or P25.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 042295040

Download citation: RISBibTeXText

PMID: 7298067


Related references

Antigens and allergens in Dermatophagoides farinae mite 1 Immunochemical and physicochemical study of two allergenic fractions from a partially purified Dermatophagoides farinae mite extract. Immunology 442: 239-247, 1981

Antigens and allergens in dermatophagoides farinae mite 1. immunochemical and physicochemical study of 2 allergenic fractions from a partially purified dermatophagoides farinae mite extract. Immunology 44(2): 239-248, 1981

Allergens of the house dust mite dermatophagoides farinae immunochemical studies of 4 allergenic fractions. Journal of Allergy & Clinical Immunology 75(6): 686-692, 1985

Allergens of the house dust mite Dermatophagoides farinae--immunochemical studies of four allergenic fractions. Journal of Allergy and Clinical Immunology 75(6): 686-692, 1985

Demonstration of close physicochemical similarity and partial immunochemical identity between the major allergen dp 42 of the house dust mite dermatophagoides pteronyssinus and corresponding antigens of dermatophagoides farinae df 6 and dermatophagoides microceras dm 6. International Archives of Allergy & Applied Immunology 79(1): 60-65, 1985

Comparison of antigenic and allergenic composition of 2 partially purified extracts from dermatophagoides farinae and dermatophagoides pteronyssinus mite cultures. Journal of Allergy & Clinical Immunology 71(6): 588-596, 1983

Comparison of antigenic and allergenic composition of two partially purified extracts from Dermatophagoïdes farinae and Dermatophagoïdes pteronyssinus mite cultures. Journal of Allergy and Clinical Immunology 71(6): 588-596, 1983

Antigens and allergens in dermatophagoides farinae mite 2. purification of ag 11 a major allergen in dermatophagoides farinae. Immunology 46(4): 679-688, 1982

Antigens and allergens in Dermatophagoïdes farinae mite. II. Purification of AG f1, a major allergen in Dermatophagoïdes farinae. Immunology 46(4): 679-687, 1982

Studies on antigens of the house-dust mite. II. Allergenic activities of polyacrylamide gel electrophoresis fractions of Dermatophagoides farinae extract. Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 1(3): 178-181, 1983

Evaluation of a purified house dust mite preparation from dermatophagoides farinae spectralgen dermatophagoides farinae in diagnostic use. Allergy 37(Suppl. 1): 36, 1982

Elisa for determination of major excrement allergens of house dust mite species dermatophagoides pteronyssinus dermatophagoides farinae and dermatophagoides microceras. Allergy 41(6): 442-451, 1986

Comparative analysis of physicochemical and immunochemical properties of the two major allergens from Dermatophagoides pteronyssinus and the corresponding allergens from Dermatophagoides farinae. International Archives of Allergy and Applied Immunology 88(4): 402-407, 1989

High-molecular-size mite allergens with high carbohydrate content in mite extract containing mite feces of Dermatophagoides farinae. Arerugi 44(3 Pt 1): 170-181, 1995

Allergens of the house dust mite Dermatophagoides farinae. II. Immunological characterization of four allergenic molecules. International Archives of Allergy and Applied Immunology 88(1-2): 173-175, 1989