+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Assignment of human natural killer (NK)-like cells to the T cell lineage. Single allospecific T cell clones lyse specific or NK-sensitive target cells via distinct recognition structures



Assignment of human natural killer (NK)-like cells to the T cell lineage. Single allospecific T cell clones lyse specific or NK-sensitive target cells via distinct recognition structures



European Journal of Immunology 14(2): 121-125



The aim of the present study was to define the cell lineage of mixed lymphocyte culture (MLC)-induced natural killer (NK) effector cells. Human MLC cells were plated under limiting microculture conditions in the presence of irradiated spleen cells and interleukin 2-containing supernatant. After 18 days, microcultures were scored for proliferation and for cytolytic activity against specific lymphoblasts and NK-sensitive K562 target cells. About 1 in 7 and 1 in 5 proliferating microcultures had specific or NK-like cytolytic activity, respectively. Moreover, several microcultures exhibited dual (specific and NK-like) cytolytic activity, even when they had been established at relatively low numbers of responding cells/well (0.5-0.25) to ensure a high probability of monoclonality. Direct evidence for the existence of cytolytic effector cells with dual activity was achieved by using clones derived from single MLC T cells by micromanipulation. Out of 26 cytolytic clones so derived, 16 exhibited specific cytolytic activity, whereas 22 lysed K562 target cells. More interestingly, 12 of these 26 clones were active against both types of target cells. Only one of these clones was able to lyse autologous or unrelated target cells. In contrast, all such clones lysed the NK-sensitive cell lines G11, MOLT-4, Raji, Daudi, Chang and T-24. Addition of saturating amounts of B9-4 monoclonal antibody in the lytic assays resulted in the inhibition of the specific cytolysis, but not the NK-like activity of clones with dual cytolytic activity. It thus appears that (a) alloreactive cytotoxic T lymphocytes can mediate both specific and NK-like cytolysis and (b) two independent recognition structures are involved in this dual activity.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 042331397

Download citation: RISBibTeXText

PMID: 6230242

DOI: 10.1002/eji.1830140204


Related references

Studies on the mechanism of natural killer cell mediated cyto toxicity 1. release of cyto toxic factors specific for natural killer sensitive target cells during co culture of natural killer effector cells with natural killer target cells. Journal of Immunology 129(1): 433-439, 1982

Natural killer (NK)-like cytotoxic activity of allospecific T cell receptor-gamma,delta+ T cell clones. Distinct receptor-ligand interactions mediate NK-like and allospecific cytotoxicity. Journal of Immunology 142(12): 4161-4168, 1989

Recognition of virus-infected cells by natural killer cell clones is controlled by polymorphic target cell elements. Journal of Experimental Medicine 178(3): 961-969, 1993

Heterogeneity of human natural killer effector cells which lyse herpes simplex virus type 1 infected fibroblasts are related to but distinct from those which lyse k 562 cells. Immunobiology-4: 403-404, 1982

Target cell spectrum of mixed lymphocyte culture activated natural killer cells and induction of natural killer target cell structures by phyto hem agglutinin. Immunobiology 159(1-2): 133-134, 1981

Natural killer-like cytotoxicity of human T-cell clones against various target cells. Scandinavian Journal of Immunology 17(1): 95-98, 1983

Expression of HLA-C molecules confers target cell resistance to some non-major histocompatibility complex-restricted T cells in a manner analogous to allospecific natural killer cells. Journal of Experimental Medicine 182(4): 1005-1018, 1995

Studies on the mechanism of natural killer cytotoxicity. II. coculture of human PBL with NK-sensitive or resistant cell lines stimulates release of natural killer cytotoxic factors (NKCF) selectively cytotoxic to NK-sensitive target cells. Journal of Immunology 130(5): 2479-2483, 1983

Identification of four subsets of human CD3-CD16+ natural killer (NK) cells by the expression of clonally distributed functional surface molecules: correlation between subset assignment of NK clones and ability to mediate specific alloantigen recognition. Journal of Experimental Medicine 172(6): 1589-1598, 1990

Induction of erythroid differentiation in K562 cells and natural killer cell-mediated lysis: distinct effects at the level of recognition and lysis in relation to target cell proliferation. Immunobiology 167(4): 389-397, 1984

CD45-negative mutants of rat natural killer cell line fail to lyse tumor target cells. Journal of Immunology 151(7): 3646-3653, 1993

Target cell structures recognized by human natural killer cells. Immunobiology 162(4-5): 401-402, 1982

Single-cell analysis of the dynamics and functional outcomes of interactions between human natural killer cells and target cells. Integrative Biology 4(10): 1175-1184, 2012

"Harpoon" model for cell-cell adhesion and recognition of target cells by the natural killer cells. Journal of Theoretical Biology 180(4): 321-342, 1996

Cell surface glycoconjugates as possible target structures for human natural killer cells. Journal of Cellular Biochemistry Suppl. 0(17 Part A): 374, 1993