Section 43
Chapter 42,384

Bilirubin interference in a peroxidase-coupled procedure for creatinine eliminated by bilirubin oxidase

Artiss, J.D.; McEnroe, R.J.; Zak, B.

Clinical Chemistry 30(8): 1389-1392


ISSN/ISBN: 0009-9147
PMID: 6744594
Accession: 042383343

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A colorimetric peroxidase-coupled procedure for determination of creatinine in human serum and urine is described. A 30-s sample pre-treatment with bilirubin oxidase eliminates interference from endogenous bilirubin. The 4-aminoantipyrine-2-hydroxy-3,5-dichlorobenzenesulfonate chromogen system of this method is about fourfold more sensitive than current procedures that involve monitoring NAD(P)H and nearly fivefold more sensitive than the traditional picrate procedures. Incorporation of a sample blank eliminates positive interference from endogenous creatine. Results of the proposed procedure are somewhat lower than those of the common kinetic or equilibrium picrate techniques, as would be expected because of the effects of the well-known interferences in the latter methods.

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