+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Binding of a rhodamine-labeled lipopolysaccharide to lipopolysaccharide-responder and nonresponder lymphocytes



Binding of a rhodamine-labeled lipopolysaccharide to lipopolysaccharide-responder and nonresponder lymphocytes



Cellular Immunology 85(2): 531-541



The binding levels of a highly soluble rhodamine-labeled lipopolysaccharide (LPS-Rh) on different LPS-responsive and nonresponsive murine spleen cells were measured with a cytofluorometer (CFM). In all the experiments, a preferential binding of LPS-Rh to LPS-responsive cells was observed. Furthermore, only a proportion of B cells from the responder mouse strain was selectively stained after addition of LPS-Rh. These findings suggested that LPS-Rh binds to specific components of the cell membrane, involved in the triggering of B-cell mitogenicity.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 042384285

Download citation: RISBibTeXText

PMID: 6201292

DOI: 10.1016/0008-8749(84)90265-x


Related references

Differential binding of a lipopolysaccharide rhodamine conjugate to lipopolysaccharide responder and nonresponder b lymphocytes. Biology Of The Cell (paris): 74a, 1984

Lipopolysaccharide receptors on lymphocytes. I. Lack of immunologic recognition of a putative LPS receptor on LPS-responder lymphocytes by LPS-nonresponder mice. Journal of Immunology 135(3): 1906-1910, 1985

Bacterial phagocytosis by macrophages from lipopolysaccharide responder and nonresponder mouse strains. Infection and Immunity 28(3): 762-765, 1980

The lipid A moiety of lipopolysaccharide is specifically bound to B cell subpopulations of responder and nonresponder animals. Journal of Immunology 125(1): 102-107, 1980

Lipopolysaccharide-responder and nonresponder C3H mouse strains are equally susceptible to an induced Escherichia coli urinary tract infection. Infection and Immunity 64(4): 1369-1372, 1996

Adaptive differentiation of murine lymphocytes. IV (Responder x nonresponder) F1 T cells can be taught to preferentially help nonresponder, rather than responder, B cells. Journal of Experimental Medicine 150(1): 20-30, 1979

Differences in lipopolysaccharide lps binding to macrophages of lps responder and low responder mice. Immunobiology 183(3-4): 327, 1991

Inhibition of activated nonresponder c3h h3j lymphocytes by lipopolysaccharide endotoxin. Infection & Immunity 56(12): 3040-3045, 1988

Inheritance of lipopolysaccharide-enhanced nonspecific resistance to infection and of susceptibility to endotoxic shock in lipopolysaccharide low-responder mice. Infection and Immunity 16(2): 432-438, 1977

Antagonistic effects of lipopolysaccharide binding protein and bactericidal/permeability-increasing protein on lipopolysaccharide-induced cytokine release by mononuclear phagocytes. Competition for binding to lipopolysaccharide. Journal of Immunology 151(8): 4258-4265, 1993

High Mobility Group Box 1 Protein Binding to Lipopolysaccharide Facilitates Transfer of Lipopolysaccharide to CD14 and Enhances Lipopolysaccharide-Mediated TNF-a Production in Human Monocytes. 2008

Electrokinetic properties of splenic lymphocytes from the low-lipopolysaccharide responder C3H/Hej mice. Folia Biologica 12(3): 145-150, 1976

High mobility group box 1 protein binding to lipopolysaccharide facilitates transfer of lipopolysaccharide to CD14 and enhances lipopolysaccharide-mediated TNF-alpha production in human monocytes. Journal of Immunology 180(7): 5067-5074, 2008

Bacterial lipopolysaccharides bind selectively to lymphocytes from lipopolysaccharide high-responder mouse strains. Scandinavian Journal of Immunology 10(6): 555-561, 1979

Surfactant protein a inhibits lipopolysaccharide-induced immune cell activation by preventing the interaction of lipopolysaccharide with lipopolysaccharide-binding protein. American Journal of Respiratory Cell and Molecular Biology 27(3): 353-360, 2002