+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterisation of viral RNA in cells infected with the murine coronavirus JHM



Characterisation of viral RNA in cells infected with the murine coronavirus JHM



Advances in Experimental Medicine and Biology 142: 91



The murine coronavirus JHM induces in Sac(-) cells seven major and two minor RNA species. These RNAs are polyadenylated and single stranded. Their sizes were estimated by electrophoresis in agarose gels containing methylmercury hydroxide. The mol. wts. for the major species are 6.67 x 10(6) for RNA of genome size, 3.42 x 10(6) for RNA 2, 2.76 x 10(6) for RNA 3, 1.35 x 10(6) for RNA 4, 1.19 x 10(6) for RNA 5, 0.93 x 10(6) for RNA 6 and 0.62 x 10(6) for RNA 7. The minor species have a size of 4.7 x 10(6) (RNA a) and 1.5 x 10(6) (RNA b). No essential difference in the number and proportion of each RNA species was found between total cytoplasmic RNA, polyadenylated cytoplasmic RNA and RNA extracted from pelleted polysomes, nor was any difference found during the infection cycle. The major RNA species are likely to be subgenomic mRNAs.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 042506712

Download citation: RISBibTeXText

PMID: 6175191


Related references

Dissociation of demyelination and viral clearance in congenitally immunodeficient mice infected with murine coronavirus JHM. Journal of Neurovirology 2(2): 101-110, 1996

Characterisation of cyclin D1 down-regulation in coronavirus infected cells. Febs Letters 581(7): 1275-1286, 2007

The murine coronavirus mouse hepatitis virus strain A59 from persistently infected murine cells exhibits an extended host range. Journal of Virology 71(12): 9499-9507, 1997

The N-terminal region of the murine coronavirus spike glycoprotein is associated with the extended host range of viruses from persistently infected murine cells. Journal of Virology 78(17): 9073-9083, 2004

Viral and Cellular mRNA Translation in Coronavirus-Infected Cells. Advances in Virus Research 96: 165-192, 2016

Specific cleavage of 28S ribosomal RNA in murine coronavirus-infected cells. Advances in Experimental Medicine and Biology 494: 621-626, 2001

RNA-dependent RNA polymerase activity in murine coronavirus-infected cells. Journal of General Virology 64: 103-111, 1983

Induction of apoptosis in murine coronavirus-infected 17Cl-1 cells. Advances in Experimental Medicine and Biology 494: 615-620, 2001

Synthesis of virus-specific RNA in permeabilized murine coronavirus-infected cells. Virology 166(1): 66-75, 1988

Identification of putative polymerase gene product in cells infected with murine coronavirus A59. Virology 157(2): 565-568, 1987

RNase L-independent specific 28S rRNA cleavage in murine coronavirus-infected cells. Journal of Virology 74(19): 8793-8802, 2000

Regulation of viral persistence in human glioblastoma and rhabdomyosarcoma cells infected with coronavirus OC43. Microbial Pathogenesis 1(6): 573-582, 1986

Viral RNA of cells infected with murine retrovirus: (II). Effect of toyocamycin on the metabolism of viral RNA. Biochimie 61(9): 1011-1019, 1979

Viral rna of murine retrovirus infected cells 2. influence of toyocamycin on viral rna metabolism. Biochimie 61(9): 1011-1020, 1979

A murine coronavirus MHV-S isolate from persistently infected cells has a leader and two consensus sequences between the M and N genes. Virology 198(1): 355-359, 1994