+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of eight forms of corticotropin-like intermediary lobe peptide from the rat intermediary pituitary



Characterization of eight forms of corticotropin-like intermediary lobe peptide from the rat intermediary pituitary



Journal of Biological Chemistry 257(17): 10096-10102



The isolation and characterization of eight forms of corticotropin-like intermediary lobe peptide (CLIP, adrenocorticotropin18-39) from the intermediary lobe of the rat pituitary has been accomplished by using reversed phase high performance liquid chromatography. The eight forms are the result of all combinations of the presence or absence of three post-translational modifications. These are glycosylation, phosphorylation, and removal of the carboxyl-terminal amino acid. The sites of phosphorylation and glycosylation are at serine 31 and asparagine 29, respectively. The eight forms (in order of elution from the reversed high performance liquid chromatography column) are glycosylated, phosphorylated CLIP18-38; glycosylated, nonphosphorylated CLIP18-38; nonglycosylated, phosphorylated CLIP18-38; nonglycosylated, nonphosphorylated CLIP18-38; glycosylated, phosphorylated CLIP18-39; glycosylated, nonphosphorylated CLIP18-39; nonglycosylated, phosphorylated CLIP18-39; and nonglycosylated, nonphosphorylated CLIP18-39.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 042513669

Download citation: RISBibTeXText

PMID: 6286638


Related references

Phosphorylated forms of adrenocorticotropin and corticotropin-like intermediary lobe peptide in human tumors. European Journal of Endocrinology 131(4): 341-346, 1994

Corticotropin like intermediary lobe peptide as a marker of alternate pro opiomelanocortin processing in acth producing non pituitary tumors. Clinical Endocrinology 31(6): 691-700, 1989

Isolation of acth 1 39 acth 1 38 and corticotropin like intermediary lobe peptide from the calf anterior pituitary. Biochemical & Biophysical Research Communications 96(3): 1441-1448, 1980

Isolation and characterization of gamma 3 melanotropin from the neuro intermediary lobe of the rat pituitary. Biochemical & Biophysical Research Communications 100(1): 336-343, 1981

Participation of the intermediary lobe in the production of interstitial substances of the pituitary gland. Revue Medicale de Nancy 78: 580-584, 1953

Comparison of adenylate cyclase activity in the neurohypophysis and the intermediary lobe of the pituitary gland of the rat. Physiologia Bohemoslovaca 34(5): 432-433, 1985

Peptides of the neuro intermediary lobe of rat pituitary stimulate growth hormone secretion in vitro. Molecular & Cellular Endocrinology 32(1): 47-56, 1983

Ultrastructural and immunocytological study of the intermediary lobe of the pituitary in a Saharan rodent Gerbillus gerbillus. Bulletin de la Societe Zoologique de France Evolution et Zoologie 121(1): 152-153, 1996

The role of the Medicare fiscal intermediary and the regional home health intermediary, Part 2. Journal of Nursing Administration 22(7-8): 24-29, 1992

The role of the Medicare fiscal intermediary and the Regional Home Health Intermediary, Part 1. Journal of Nursing Administration 22(6): 47-53, 1992

Corticotropin like intermediate lobe peptide a new pituitary and tumor peptide. Journal Of Endocrinology: Xxxvi-Xxxvii, 1972

Corticotropin like intermediate lobe peptide in the salmon pituitary. Nippon Suisan Gakkaishi 46(6): 743-747, 1980

Corticotropin like intermediate lobe peptide in the salmon oncorhynchus keta pituitary. Nippon Suisan Gakkaishi 46(6): 743-748, 1980

Occurrence of a new corticotropin-like intermediate lobe peptide in salmon pituitary glands. Archives of Biochemistry and Biophysics 213(2): 680-688, 1982

Clinical Studies on Intermediary Metabolism of Tyrosine. 2. Urinary Excretion of Intermediary Metabolites of Tyrosine in Patients with Blood Disorders. Naika Hokan. Japanese Archives of Internal Medicine 11: 97-109, 1964