EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Charge-shift probes of membrane potential: a probable electrochromic mechanism for p-aminostyrylpyridinium probes on a hemispherical lipid bilayer



Charge-shift probes of membrane potential: a probable electrochromic mechanism for p-aminostyrylpyridinium probes on a hemispherical lipid bilayer



Biophysical Journal 34(3): 353-365



The characteristics of the spectroscopic responses to membrane potential are examined for a series of dyes based on the 4-(p-aminostyryl)-1-pyridinium chromophore. An apparatus using an oxidized cholesterol hemispherical bilayer and phase-sensitive detection provides response spectra in either transmission or fluorescence excitation modes. All the probes with good binding properties display biphasic response spectra that are similar in both shape and magnitude. Detailed analysis of the response spectra allows all the previously discovered mechanisms for extrinsic potential sensitive molecular probes, which require a change in the probe's chemical environment, to be ruled out. The data are consistent with an electrochromic mechanism. Polarized fluorescence intensities from the membrane-bound probes indicate that the chromophore is optimally oriented for an electrochromic response.

(PDF same-day service: $19.90)

Accession: 042516913

Download citation: RISBibTeXText

PMID: 7248466

DOI: 10.1016/S0006-3495(81)84854-0



Related references

Charge-shift probes of membrane potential. Characterization of aminostyrylpyridinium dyes on the squid giant axon. Biophysical Journal 47(1): 71-77, 1985

New charge shift probes of membrane potential. Federation Proceedings 39(6): ABSTRACT 2758, 1980

Evidence for a charge-shift electrochromic mechanism in a probe of membrane potential. Nature 281(5731): 497-499, 1979

Charge shift probes of membrane potential synthesis. Journal of Organic Chemistry 49(14): 2546-2551, 1984

Charge shift optical probes of membrane potential. Theory. Biochemistry 17(19): 4065-4071, 1978

Emission response spectra for charge shift probes of membrane potential. Biophysical Journal 33(2 PART 2): 114A, 1981

Design of electrochromic probes of membrane potential. Biophysical Journal 21(3): 206A, 1978

Redistribution of lipid probes at early stages of bilayer lipid membrane fusion. Membrane & Cell Biology 10(6): 649-656, 1997

Interaction of charged lipid vesicles with planar bilayer lipid membranes: detection by antibiotic membrane probes. Journal of Supramolecular Structure 5(3): 409-416, 1976

Spectra membrane binding and potentiometric responses of new charge shift probes. Biochemistry 24(21): 5749-5755, 1985

Membrane binding flip flop and voltage dependent spectroscopy of charge shift probes. Biophysical Journal 45(2 PART 2): 167A, 1984

Lipid bilayer regulation of membrane protein function: gramicidin channels as molecular force probes. Journal of the Royal Society, Interface 7(44): 373-395, 2010

Spectral properties of fluorescent dyes in lecithin vesicles. Probes for the structure of lipid bilayer membranes and for membrane potentials. Zeitschrift für Naturforschung. Section C, Biosciences 31(9-10): 575-588, 1976

Electrochromic properties of membrane probes. Biomembranes 7: 56-60, 1975

Determination of the electric potential at the external and internal bilayer-aqueous interfaces of the human erythrocyte membrane using spin probes. Biochimica et Biophysica Acta 732(3): 683-690, 1983