+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Chromosome mutation tests for mutagenesis in Drosophila melanogaster. A report of the U.S. Environmental Protection Agency Gene-Tox Program



Chromosome mutation tests for mutagenesis in Drosophila melanogaster. A report of the U.S. Environmental Protection Agency Gene-Tox Program



Mutation Research 134(1): 61-88



The term 'chromosome mutations' was chosen and defined for this review to refer to alterations of chromosome structure (reciprocal, heritable translocations), of chromosome number (loss or gain of a whole chromosome), or of chromosome content (loss or gain of a part of a chromosome). Chromosome mutations may result from chromosome breakage (clastogenesis) and its consequences or from disruption of chromosome behavior during cell division (nondisjunction). State-of-the-art protocols are outlined to test for heritable translocations, for whole-or partial chromosome loss (clastogenesis), and for whole chromosome loss or gain (nondisjunction). The literature up to 1980 was reviewed and 106 papers were selected for the evaluation of 116 chemicals for one or more chromosome mutation end points. The criteria used for acceptance of data from the literature were not stringent, as most of this work was done some time ago and for purposes other than testing. The main criterion was that germ cell stage sampling was correct. For the evaluation of the accepted data, numerical requirements were set up, using as a guide the control data from all the papers. Compounds were classified, when possible, as mutagenic (+) or nonmutagenic (-). Those not classifiable, usually due to insufficient numbers of chromosomes tested, were listed as inconclusive (inc). Of 61 compounds tested for heritable translocations, 27 were positive, 8 were negative, and 26 were inconclusive. Of the 35 with conclusive data, only 21 also have definitive carcinogenesis classifications (all positive). Of these, 19 were deemed mutagenic, which gives agreement of 90.5%. Of the 76 compounds tested for clastogenesis by the chromosome loss test, 26 were positive, 13 were negative, and 37 were inconclusive. Of the 39 with conclusive data, only 20 also have definitive carcinogenesis classifications. 15 of the 19 carcinogens were positive. Four of the carcinogens were negative and 1 noncarcinogen was positive, for an overall agreement of 75%. Of 44 compounds tested for nondisjunction, 15 were positive, 13 were negative, 16 were inconclusive. Of the 28 compounds with conclusive data, only 9 have definitive carcinogenesis classifications (all positive). Five of these were deemed negative and agreement was only 44%. It should be noted that these data do not fairly represent these short-term tests as conducted with current protocols. A more equitable comparison could be achieved with planned experiments that include the sex-linked recessive lethal (SLRL) test in the comparison.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 042535930

Download citation: RISBibTeXText

PMID: 6431279

DOI: 10.1016/0165-1110(84)90015-0


Related references

The sex-linked recessive lethal test for mutagenesis in Drosophila melanogaster. A report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutation Research 123(2): 183-279, 1983

The sex-linked recessive lethal test for mutagenesis in Drosophila melanogaster--a report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutation research: Reviews in genetic toxicology 123(2): 183-279, 1983

Mutation tests in Neurospora crassa--a report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutation research: Reviews in genetic toxicology 133(2): 87-134, 1984

Mutation tests in Neurospora crassa. A report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutation Research 133(2): 87, 1984

Dna repair assays as tests for environmental mutagens a report of the usa environmental protection agency gene tox program. Mutation Research: 287-318, 1982

Vicia cytogenetic tests for environmental mutagens. A report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutation Research 99(3): 257-271, 1982

Unscheduled DNA synthesis tests. A report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutation Research 123(3): 363-410, 1983

Specific-locus mutation assay in Zea may: A report of the U.S. environmental protection agency Gene-Tox program. Mutation Research/Reviews in Genetic Toxicology 99(3): 317-337, 1982

Chromosome aberration assays in Allium. A report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutation Research 99(3): 273-291, 1982

Specific-locus mutation assays in Zea mays. A report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutation Research 99(3): 317-337, 1982

The L5178Y/tk+/- mouse lymphoma specific gene and chromosomal mutation assay a phase III report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutation Research 394(1-3): 177-303, 1997

The L5178Y/tk+/- mouse lymphoma specific gene and chromosomal mutation assay. A phase III report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutation Research 394(1-3): 177-303, 1997

Chromosome aberration assays in barley (Hordeum vulgare) a report of the U.S. environmental protection agency Gene-Tox program. Mutation Research/Reviews in Genetic Toxicology 99(1): 13-36, 1982

Chromosome aberration assays in barley hordeum vulgare a report of the usa environmental protection agency gene tox program. Mutation Research 99(1): 13-36, 1982

An evaluation of the mouse sperm morphology test and other sperm tests in nonhuman mammals. A report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutation Research 115(1): 1-72, 1983