+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comparative analysis of UV-induced mutability of ten different codon units in position 211 of the Escherichia coli trpA gene



Comparative analysis of UV-induced mutability of ten different codon units in position 211 of the Escherichia coli trpA gene



Molecular and General Genetics 196(3): 530-532



To study the influence of the local base composition upon UV-induced mutability the reversion frequencies of ten trpA mutants with known codon sequences at position 211 were compared. Comparison of mutant strains reverting by the same base substitution type but with different dipyrimidinic sequences reveals the mutagenic character of the pyrimidine-pyrimidine (6-4) photoproduct. The codons GAC and GAT, both reverting by AT-GC transitions and AT-CG transversions in the middle position and harboring the dipyrimidinic sequence CT in the opposite strand, differ in their reversion frequencies sixfold. This difference can only be due to the influence of the different bases in the third codon position upon the mutability of adjacent second bases.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 042606331

Download citation: RISBibTeXText

PMID: 6094982


Related references

In vitro mutagenesis of the escherichia coli trpa gene and the expression and properties of corresponding mutant trpa alpha subunits. Federation Proceedings 45(6): 1929, 1986

Sequence verification of mutant codon assignments in trpA of Escherichia coli. Dna 5(2): 123-128, 1986

Uv induced tandem double mutations in the trpa gene of escherichia coli. Studia Biophysica 80(3): 231-232, 1980

Rare codon priority and its position specificity at the 5' of the gene modulates heterologous protein expression in Escherichia coli. Biochemical and Biophysical Research Communications 376(4): 647-652, 2008

A molecular characterization of spontaneous frameshift mutagenesis within the trpA gene of Escherichia coli. Dna Repair 6(2): 177-189, 2007

Primary structure of escherichia coli leucine transfer rna uur presence of an unknown adenosine derivative in the 1st position of the anti codon which recognizes the uu codon series. Journal of Biological Chemistry 255(5): 2220-2223, 1980

Change of spontaneous and uv induced mutability in streptomycin mutants of escherichia coli depending on the allelic state of rpsl gene. Biologicheskii Zhurnal Armenii 31(3): 259-263, 1978

A comparative study of variation in codon 33 of the rpoS gene in Escherichia coli K12 stocks: implications for the synthesis of ss. Molecular Genetics and Genomics 270(6): 3-8, 2004

A comparative study of variation in codon 33 of the rpoS gene in Escherichia coli K12 stocks: implications for the synthesis of sigma(s). Molecular Genetics and Genomics 270(6): 533-538, 2004

A comparative study of mutations in Escherichia coli and Salmonella typhimurium shows that codon conservation is strongly correlated with codon usage. Journal of Theoretical Biology 206(2): 307-311, 2000

Influence of the codon following the AUG initiation codon on the expression of a modified lacZ gene in Escherichia coli. Embo Journal 6(8): 2489-2492, 1987

Presumed anti codon structure of glutamic acid transfer rna from escherichia coli a possible location of a 2 thio uridine derivative in the 1st position of the anti codon. Biochemical & Biophysical Research Communications 40(4): 866-872, 1970

In vitro mutagenesis and overexpression of the Escherichia coli trpA gene and the partial characterization of the resultant tryptophan synthase mutant a-subunits. The Journal of Biological Chemistry 261: 604-15, 1986

Expression of a Chimeric Allergen with High Rare Codons Content in Codon Bias-Adjusted Escherichia coli: Escherichia coli BL21 (DE3)-Codon Plus RIL as an Efficient Host. Current Microbiology 73(1): 91-98, 2016

In vitro mutagenesis and overexpression of the Escherichia coli trpA gene and the partial characterization of the resultant tryptophan synthase mutant alpha-subunits. Journal of Biological Chemistry 261(35): 16604-16615, 1986