Considerations on the value of freeze-etching technique in studying the ultrastructure of some anaerobic bacteria

Vaisman, I.S.

Acta Histochemica. Supplementband 23: 241-247


ISSN/ISBN: 0567-7556
PMID: 6261293
Accession: 042660605

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Samples from periodic cultures of Clostridium perfringens, Clostridium oedematiens, Clostridium tetani and Bifidobacterium bifidum grown in liquid nutrient media were studied after freeze-fracturing and etching without pretreatment by means of chemical agents including cryo-protectants. Subunits in the cell all of Cl. tetani and differences in the distribution of globular particles in the plasma membrane of the investigated bacteria are revealed.