+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Control of latent allotype expression by rabbit splenocytes

Control of latent allotype expression by rabbit splenocytes

Journal of Immunology 127(1): 352-357

Serologic and chemical studies have suggested that immunoglobulin structural genes are under nonallelic genetic control. The transient appearance of unexpected (i.e., latent) allotypes in serum further suggests that cellular control mechanisms may be operative. We report here the results of our efforts to induce the expression of latent allotypes by cultured rabbit splenocytes. The protein A plaque assay facilitated with various anti-allotypic antisera was used to enumerate allotype-specific plaque-forming cells. When splenocytes from a b4 b4 rabbit are cultured in the presence of anti-b4 alloantisera, the number of b4 PFC (i.e., nominal) is suppressed and the number of PFC expressing the latent allotype is increased. The specificity of the induced latent PFC is dependent upon the source and allotypic mosaic of the antiserum added to the cultures. Our results suggest that lymphocytes committed to latent allotypes are normally suppressed. Activation by the appropriate alloantiserum directed against the nominal allotype may relieve a cell-mediated suppressive mechanism.

(PDF emailed within 1 workday: $29.90)

Accession: 042677042

Download citation: RISBibTeXText

PMID: 7016994

Related references

Latent allotype expression in the rabbit. Dubiski, S (Ed ) Monographs And Surveys in The Biosciences: The Rabbit in Contemporary Immunological Research Xv+206p John Wiley And Sons, Inc : Somerset, New Jersey, Usa; Longman Scientific And Technical, Ltd : London, England, Uk Illus Maps 134-147, 1987

Identification of rabbit genomic Ig-VH pseudogenes that could serve as donor sequences for latent allotype expression. Journal of Immunology 145(8): 2713-2717, 1990

Restriction on the secretion of hemolytic antibodies of more than 1 allotype by rabbit splenocytes. Archives de l'Institut Pasteur d'Algerie (50-51): (1975) 131-138, 1972, 1973

Restriction on the secretion of hemolytic antibodies of more than one allotype by rabbit splenocytes. Archives 73 (Pub 1975), 50 51 131-138, 1975

Restriction on the secretion of hemolytic antibodies of more than 1 allotype by individual rabbit splenocytes. Federation Proceedings 29(2): 768, 1970

Expression of cell-surface latent allotypes: latent specificities are synthesized only by cells that display the nominal allotype. Journal of Immunology 126(6): 2490-2493, 1981

Comparison of latent and nominal rabbit ig vha1 allotype complementary dna sequences. Journal of Immunology 141(6): 2063-2071, 1988

Genomic v h regions in rabbit not typical of any one v ha allotype could account for some observations of latent allotypes. Federation Proceedings 44(3): 553, 1985

3 closely linked latent rabbit immuno globulin g allotype gene products a 1 d 12 e 14. Journal of Immunology 131(4): 1860-1864, 1983

Identification of rabbit immunoglobulin latent Ckappa1 allotype genes alters the concept of allelic inheritance. Molecular Immunology 35(14-15): 965-976, 1999

Expression of latent allotype in a stock of sjl mice. Journal of Supramolecular Structure 8(SUPPL 3): 312, 1979

A genomic gene encoding the b5 rabbit immunoglobulin kappa constant region: implications for latent allotype phenomenon. Proceedings of the National Academy of Sciences of the United States of America 81(6): 1789-1793, 1984

Enhancement in Igha mouse strains of the "natural" suppressive activity of normal T splenocytes against the expression of Igh-1b allotype. I. Molecular aspects of the chronic suppression obtained. European Journal of Immunology 18(1): 51-58, 1988

Switched allotype expression in an immunoglobulin-nonsecreting rabbit lymphoid cell line fused with rabbit gangliocytes. European Journal of Immunology 11(5): 388-392, 1981

Rabbit latent vhal allotype sequences comparison of amino acid and complementary dna sequences. Journal of Cellular Biochemistry Supplement (12 PART B): 99, 1988