+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Coronavirus: how a large RNA viral genome is replicated and transcribed

Coronavirus: how a large RNA viral genome is replicated and transcribed


Coronaviruses are important human and animal pathogens and contain an extraordinarily long (27-31 kb) RNA genome. Its RNA synthesis involves complex mechanisms of regulation, similar to those of DNA viruses. In this treatise, mouse hepatitis virus (MHV) is used as a model for the discussion of the mechanism of viral RNA synthesis. We show that MHV RNA synthesis requires interactions of multiple RNA components, which are likely mediated by protein-RNA and protein-protein, as well as RNA-RNA, interactions. This virus also provides a unique example of a discontinuous transcription mechanism, which involves a trans-acting RNA component. Finally, study of the cis-acting signals for the various steps of RNA synthesis revealed an insight into the regulation of viral RNA synthesis. This discussion suggests that the regulation of RNA synthesis in coronavirus is more complex than previously thought possible for RNA viruses. Coronavirus RNA transcription and replication may serve as a paradigm of RNA synthesis for RNA viruses in general.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 042685776

Download citation: RISBibTeXText

PMID: 7812660

Related references

Sequence analysis of the 3'-end of the feline coronavirus FIPV 79-1146 genome: comparison with the genome of porcine coronavirus TGEV reveals large insertions. Virology 167(2): 370-376, 1988

Infectious RNA transcribed in vitro from a cDNA copy of the human coronavirus genome cloned in vaccinia virus. Journal of General Virology 82(Pt 6): 1273-1281, 2001

Transmissible gastroenteritis coronavirus genome packaging signal is located at the 5' end of the genome and promotes viral RNA incorporation into virions in a replication-independent process. Journal of Virology 87(21): 11579-11590, 2013

Interaction of coronavirus nucleocapsid protein with the 5'- and 3'-ends of the coronavirus genome is involved in genome circularization and negative-strand RNA synthesis. Febs Journal 286(16): 3222-3239, 2019

Characterization of viral proteins encoded by the SARS-coronavirus genome. Antiviral Research 65(2): 69-78, 2005

Gene variants associated with schizophrenia in a Norwegian genome-wide study are replicated in a large European cohort. Journal of Psychiatric Research 44(12): 748-753, 2010

A method for determining the fraction of the viral genome transcribed during infection and its application to adenovirus-infected cells. Proceedings of the National Academy of Sciences of the United States of America 60(3): 959-966, 1968

Assignment of viral proteins to the 3 double stranded rna segments of bacterio phage phi 6 genome translation of phi 6 messenger rna transcribed in vitro. Molecular & General Genetics 180(2): 385-390, 1980

Assignment of viral proteins to the three double-stranded RNA segments of bacteriophage 6 genome: Translation of 6 messenger RNAs transcribed in vitro. Molecular Genetics and Genomics 180(2): 385-389, 1980

Assignment of viral proteins to the three double-stranded RNA segments of bacteriophage phi 6 genome: translation of phi 6 messenger RNAs transcribed in vitro. Molecular and General Genetics 180(2): 385-389, 1980

Construction of a large-scale physical map of the human genome. Polymorphism of transcribed segments of ribosomal RNA genes. Molekuliarnaia Biologiia 33(3): 433-441, 1999

Construction of large scale physical map of human genome: Polymorphism in the transcribed region of ribosomal RNA genes. Molekulyarnaya Biologiya 33(3): 433-441, May-June, 1999

Large proportion of genes in one cryptic WO prophage genome are actively and sex-specifically transcribed in a fig wasp species. Bmc Genomics 15: 893, 2014

RNA sequences of opposite polarities transcribed from a large part of vaccinia virus genome are accumulated in the cytoplasm. Acta Virologica 24(6): 451-454, 1980

Rhabdovirus-like endogenous viral elements in the genome of Spodoptera frugiperda insect cells are actively transcribed: Implications for adventitious virus detection. Biologicals 44(4): 219-225, 2016