+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies



Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies



Cell 30(2): 361-372



We have prepared three monoclonal antibodies against human epidermal keratins. These antibodies were highly specific for keratins and, in combination, recognized all major epidermal keratins of several mammalian species. We have used these antibodies to study the tissue distribution of epidermis-related keratins. In various mammalian epithelia, the antibodies recognized seven classes of keratins defined by their immunological reactivity and size. The 40, 46 and 52 kilodalton (kd) keratin classes were present in almost all epithelia; the 50 kd and 58 kd keratin classes were detected in all stratified squamous epithelia, but not in any simple epithelia; and the 56 kd and 65-67 kd keratin classes were unique to keratinized epidermis. Thus the expression of specific keratin classes appeared to correlate with different types of epithelial differentiation (simple versus stratified; keratinized versus nonkeratinized).

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 042690189

Download citation: RISBibTeXText

PMID: 6183000

DOI: 10.1016/0092-8674(82)90234-3


Related references

Monoclonal antibody studies of mammalian epithelial keratins: a review. Annals of the new York Academy of Sciences 455: 307-329, 1985

Monoclonal antibody analysis of bovine epithelial keratins. Specific pairs as defined by coexpression. Journal of Biological Chemistry 261(10): 4646-4654, 1986

Monoclonal antibody studies of gingival epithelial differentiation. Journal of Dental Research 67(SPEC ISSUE MAR): 162, 1988

Epithelial cell heterogeneity in mammalian thymus: monoclonal antibody to high molecular weight keratins exclusively binds to Hassall's corpuscles. Histochemical Journal 21(6): 357-364, 1989

Gene expression of differentiation-specific keratins in oral epithelial dysplasia and squamous cell carcinoma. Oral Oncology 37(3): 251-261, 2001

Mouse-specific antibody responses to a monoclonal antibody during repeated immunoscintigraphy investigations: comparison of antibody titres and imaging studies in a rat model. European Journal of Nuclear Medicine 17(5): 216-222, 1990

Expression of a corneal specific keratin during in vivo and in vitro corneal epithelial differentiation mono clonal antibody studies. Journal of Cell Biology 97(5 Part 2): 76A, 1983

A novel monoclonal antibody against human keratins. Shi Yan Sheng Wu Xue Bao 23(4): 487-493, 1990

Epithelial cell membrane specific, novel monoclonal antibody KA-1. Journal Of Dermatological Science. 5(2): 104-113, 1993

Hair keratins and hair follicle-specific epithelial keratins. Methods in Cell Biology 78: 413-451, 2004

Monoclonal antibody Br4 recognizes specific neuronal cell types. Journal of Neuroscience Research 29(1): 107-113, 1991

The use of aIF, AE1, and AE3 monoclonal antibodies for the identification and classification of mammalian epithelial keratins. Differentiation; Research in Biological Diversity 28(1): 30-35, 1984

Isolation and characterization of a monoclonal antibody specific for epithelial cells. Cancer Research 45(2): 783-790, 1985

A monoclonal antibody specific for diploid epithelial cells in Drosophila. Nature 285(5764): 403-405, 1980

Keratins as biochemical markers of epithelial differentiation. Trends in Genetics 4(10): 277-281, 1988