+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Determination of cholesteryl esters and of cholesteryl and epicholesteryl silyl ethers by capillary gas chromatography



Determination of cholesteryl esters and of cholesteryl and epicholesteryl silyl ethers by capillary gas chromatography



Lipids 17(6): 464-468



The capillary gas chromatography of cholesteryl esters after splitless injection into a 25-m, OV-1-coated, fused silica WCOT column and a 7-m Silar 10C-coated glass WCOT column is reported. The nonpolar OV-1 column separated the cholesteryl esters principally on the basis of carbon number, but separation of the saturated esters from the unsaturated esters was also achieved. The polar Silar 10C column separated the esters mainly according to the degree of unsaturation. Thus, the 2 column types complement each other in the analysis of nanogram quantities of cholesteryl esters from small samples, such as those from plasma or single arterial atherosclerosis lesions. This technique therefore obviates some of the difficulties of analyzing such cholesteryl ester samples in the form of methyl esters (incomplete transmethylation, and contamination by solvent impurities and/or plasticizer esters). Both columns were also found to be useful for the separation and quantitation of the t-BDMS ethers of cholesterol and epicholesterol in mixtures containing various proportions of these epimers.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 042773678

Download citation: RISBibTeXText

PMID: 7109858

DOI: 10.1007/bf02535228


Related references

Radiolabeled cholesteryl ethers trace LDL cholesteryl esters but not HDL cholesteryl esters in the rat. Atherosclerosis 112(1): 1-6, 1995

Determination of plasma free fatty acids free cholesterol cholesteryl esters and triacylglycerols directly from total lipid extract by capillary gas chromatography. Analytical Biochemistry 186(2): 243-250, 1990

A plasma membrane pool of cholesteryl esters that may mediate the selective uptake of cholesteryl esters from high-density lipoproteins. Biochimica et Biophysica Acta 1002(3): 365-375, 1989

An improved method for quantification of cholesterol and cholesteryl esters in human monocyte-derived macrophages by high performance liquid chromatography with identification of unassigned cholesteryl ester species by means of secondary ion mass spectrometry. Journal of Lipid Research 38(2): 401-409, 1997

Analysis of cholesterol and cholesteryl esters in human serum using capillary supercritical fluid chromatography. Journal Of Chromatography B Biomedical Applications. 655(1): 1-8, 1994

Capillary gas chromatography/mass spectrometry of cholesteryl esters with negative ammonia chemical ionization. Biomedical and Environmental Mass Spectrometry 14(3): 131-140, 1987

Selective uptake of cholesteryl esters of low-density lipoproteins is mediated by the lipoprotein-binding site in HepG2 cells and is followed by the hydrolysis of cholesteryl esters. Biochemical Journal 318: 841-847, 1996

Selective uptake of cholesteryl esters of low-density lipoproteins is mediated by to the lipoprotein-binding site in HepG2 cell and is followed by the hydrolysis of cholesteryl esters. Biochemical Journal 318(3): 841-847, 1996

A pool of reversibly cell-associated cholesteryl esters involved in the selective uptake of cholesteryl esters from high-density lipoproteins by Hep G2 hepatoma cells. Biochimica Et Biophysica Acta. 1166(2-3): 275-283, 1993

Metabolism of cholesteryl esters and relation to diet in normal conditions and in primary, secondary and tertiary malnutrition. 1. Indices of normality of cholesteryl esters in human serum. Bollettino della Societa Italiana di Biologia Sperimentale 57(18, bis): 151, 1981

Incomplete hydrolysis of cholesteryl esters during the enzymatic cholesterol determination as evidenced by aqueous cholesteryl ester solutions comparison of 6 enzymatic procedures with the liebermann burchard method. Journal of Clinical Chemistry & Clinical Biochemistry 18(10): 595-602, 1980

Incomplete hydrolysis of cholesteryl esters during the enzymatic cholesterol determination as evidenced by aqueous cholesteryl ester solutions: comparison of six enzymatic procedures with the Liebermann-Burchard method. Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift für Klinische Chemie und Klinische Biochemie 18(10): 595-601, 1980

The semi-quantitative determination of cholesterol and cholesteryl esters by paper chromatography. Experientia 13(6): 242-242, 1957

Incorporation of defined cholesteryl esters into lipoproteins using cholesteryl ester-rich microemulsions. Journal of Biological Chemistry 257(1): 330-335, 1982

Carbon 13 nmr studies of cholesteryl esters and cholesteryl ester tri glyceride mixtures. Journal of Biological Chemistry 252(22): 8071-8080, 1977