+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Diagnosis of bovine coronaviruses using mouse erythrocyte suspensions stabilized with formalin



Diagnosis of bovine coronaviruses using mouse erythrocyte suspensions stabilized with formalin



Veterinarni Medicina 27(1): 1-10



Bovine coronavirus isolated from calf faeces diseased with gastroenteritis and passaged to colostrum-free calves agglutinated mouse and rat erythrocytes. The agglutination reaction depended on temperature and took place only at a temperature of 4 degrees C. At a temperature of 37 degrees C the agglutinate broke down within 15 minutes. The coronavirus could be detected by the haemagglutination test in the contents of the small and large intestines and in the faeces of experimentally and naturally infected calves. The agglutination capacity of mouse erythrocytes was not affected by careful fixation of these erythrocytes with formalin and subsequent lyophilization and remained unchanged for as long as 52 weeks of storage at a temperature of 4 degrees C. It was demonstrated by a comparative examination of 182 samples of the faeces of calves suffering from diarrhoea that haemagglutination test was as sensitive as electron microscopy.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 042797333

Download citation: RISBibTeXText

PMID: 6278692


Related references

Diagnostics of bovine coronavirus by means of mouse erythrocyte suspensions stabilized with formalin. Veterinarni medicina Ceskoslovenska akademie zemedelska Ustav vedeckotechnickych informaci pro zemedelstvi 27(1): 1-10, 1982

Isolation and diagnosis of coronaviruses as a factor in bovine shipping fever, and a cell line for culturing both those and other bovine coronaviruses. Official Gazette of the United States Patent & Trademark Office Patents 1193(1): 422, 1996

Antigenic variation among bovine enteric coronaviruses (BECV) and bovine respiratory coronaviruses (BRCV) detected using monoclonal antibodies. Archives of Virology 144(12): 2441-2447, 1999

Isolation of bovine respiratory coronaviruses from feedlot cattle and comparison of their biological and antigenic properties with bovine enteric coronaviruses. American Journal of Veterinary Research 60(10): 1227-1233, 1999

Bovine-like coronaviruses isolated from four species of captive wild ruminants are homologous to bovine coronaviruses, based on complete genomic sequences. Journal of Virology 82(24): 12422-12431, 2008

Antigenic and genomic relatedness of turkey-origin coronaviruses, bovine coronaviruses, and infectious bronchitis virus of chickens. Avian diseases 45(4): 978-984, 2001

The use of bovine erythrocyte suspensions for circulatory experiments in cat. Helvetica Physiologica et Pharmacologica Acta 12(2): 90-97, 1954

Stabilized suspensions of P. tularensis for sero-diagnosis of tularemia. Compt Rend Soc Biol 143(11/12): 833-834, 1949

Nucleotide and predicted amino acid sequences of all genes encoded by the 3' genomic portion (9.5 kb) of respiratory bovine coronaviruses and comparisons among respiratory and enteric coronaviruses. Virus Genes 17(1): 33-42, 1998

Diagnosis of encephalitic bovine herpesvirus type 5 (BHV-5) infection in cattle: virus isolation and immunohistochemical detection of antigen in formalin-fixed bovine brain tissues. Journal of Veterinary Diagnostic Investigation 7(2): 247-251, 1995

Physico-chemical and rheological studies of inorganic suspensions. I. Thixotropy of stabilized suspensions. Acta Poloniae Pharmaceutica 35(1): 93-98, 1978

Simplification of the hematological diagnosis of bovine leukosis. I. Addition of formalin to saponin for erythrocytolysis. Dtw. Deutsche Tierarztliche Wochenschrift 78(6): 190-193, 1971

Stimulation of DNA synthesis in cultures of mouse spleen cell suspensions by bovine transferrin. Experimental Cell Research 54(2): 195-200, 1969

The effects of some tumor markers on human erythrocyte (HCA-I and HCA-II), bovine erythrocyte (BCA) and bovine lung (CA-IV) carbonic anhydrase enzyme activities in vitro. Bratislavske Lekarske Listy 116(5): 330-333, 2015

Comparative studies on ACD-whole preserved blood, erythrocyte concentration and erythrocyte suspensions. Folia Haematologica 85(2): 149-156, 1966