+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Differentiation of neisseriaceae by isoenzyme electrophoresis



Differentiation of neisseriaceae by isoenzyme electrophoresis



Journal of Infectious Diseases 147(2): 247-251



Analysis of 71 strains of Neisseriaceae by starch-gel electrophoresis of hexokinase, phosphoglucomutase, glucose phosphate isomerase, and L-malate-nicotinamide adenine dinucleotide phosphate oxidoreductase showed that all gonococci and all memingococci have a characteristic hexokinase isoenzyme that is specific for each species and clearly distinguishes meningococci and gonococci from each other and from other species of Neisseriaceae. Strains of gonococci that were transformed into maltose utilizers by DNA from Neisseria lactamica and Neisseria meningitidis showed no change in the isoenzymes so that they could still be differentiated from meningococci and other Neisseriaceae by isoenzyme electrophoresis. In view of the limited sensitivity and specificity of conventional tests for the identification of gonococci and the possibility that gonococci may be transformed into maltose utilizers by DNA from normal throat flora, electrophoresis of hexokinase isoenzymes should be useful for the precise laboratory identification of the pathogenic neisseriae, especially those from atypical sites and those giving indeterminate reactions.

(PDF emailed within 0-6 h: $19.90)

Accession: 042819272

Download citation: RISBibTeXText

PMID: 6219168

DOI: 10.1093/infdis/147.2.247


Related references

Besnoitia strain differentiation using isoenzyme electrophoresis. Journal of Parasitology 72(3): 475-476, 1986

Differentiation of Cryptococcus neoformans serotypes by isoenzyme electrophoresis. American Journal of Clinical Pathology 86(2): 204-208, 1986

Differentiation between Naegleria fowleri and N. lovaniensis using isoenzyme electrophoresis of aspartate aminotransferase. Transactions of the Royal Society of Tropical Medicine and Hygiene 78(4): 562-563, 1984

Macro creatine kinases: results of isoenzyme electrophoresis and differentiation of the immunoglobulin-bound type by radioassay. Clinical Chemistry 28(4 Pt 1): 618-623, 1982

Standardisation of isoenzyme assays with special reference to lactate dehydrogenase isoenzyme electrophoresis. Clinical Biochemistry 7(1): 29-40, 1974

The disc electrophoresis observations on esterase isoenzyme and peroxidase isoenzyme of tissues of giant panda. Sichuan Daxue Xuebao (Ziran Kexueban): 281: 128-133, 1991

Unexpected creatine kinase isoenzyme patterns: isoenzyme electrophoresis may yield ambiguous results. Clinical Chemistry 28(8): 1824-1825, 1982

Identification of species of neisseriaceae family by the counter immuno electrophoresis inhibition method. Laboratornoe Delo: 3-266, 1979

The differentiation of invasive and non-invasive Entamoeba histolytica by isoenzyme electrophoresis. Transactions of the Royal Society of Tropical Medicine and Hygiene 72(5): 519-521, 1978

Differentiation of some species of Neisseriaceae and other bacterial groups by DNA-DNA hybridization. Apmis 97(5): 395-405, 1989

Molecular diagnostics of Neisseriaceae - Ribosomal differentiation of medical microorganisms. Abstracts of the General Meeting of the American Society for Microbiology 99: 203, 1999

Polyacrylamide gradient gel electrophoresis a routine method for high resolution isoenzyme electrophoresis of solanum and lycopersicon species. Euphytica 40(3): 181-186, 1989

Adenylate kinase mimics creatine kinase-MM isoenzyme in a CK isoenzyme electrophoresis assay. Journal Of Clinical Laboratory Analysis. 8(3): 140-143, 1994