Distribution of lecithin-cholesterol acyltransferase (LCAT) in human plasma lipoprotein fractions. Evidence for the association of active LCAT with low density lipoproteins
Chen, C.H.; Albers, J.J.
Biochemical and Biophysical Research Communications 107(3): 1091-1096
ISSN/ISBN: 0006-291X PMID: 7138515 DOI: 10.1016/0006-291x(82)90633-7
The distribution of lecithin-cholesterol acyltransferase (LCAT) in human plasma was assessed by measuring both Lcat mass and activity in plasma fractions separated by sequential flotation ultracentrifugation, single-spin gradient ultracentrifugation, dextran sulfate-Mg2+ precipitation or agarose gel filtration. Although most of the Lcat was found to be associated with the high density lipoprotein fraction, a small amount of active Lcat (approximately 1% of the plasma Lcat mass and activity) was consistently associated with the low density lipoprotein fraction. Lcat was not found in the very low density lipoprotein fraction. The LDL-associated Lcat may play an important role in the acylation of lysolecithin by lysolecithin acyltransferase activity of LCAT.