+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Dynamic states of phospholipids in Escherichia coli B membrane. Electron spin resonance studies with biosynthetically generated phospholipid spin labels



Dynamic states of phospholipids in Escherichia coli B membrane. Electron spin resonance studies with biosynthetically generated phospholipid spin labels



Biochimica et Biophysica Acta 646(1): 119-125



Mobility of phospholipid hydrocarbons in the Escherichia coli B membrane fractions was studied by labeling phosphatidylethanolamine or phosphatidylglycerol in situ by biosynthetic incorporation of the spin label. For this purpose, CDP-diacylglycerol spin label was synthesized from phosphatidic acid spin label and cytidine 5'-phosphoromorpholidate and purified by thin-layer chromatography. DCP-diacylglycerol spin label was then incorporated into phospholipids biosynthetically. ESR spectra of these E. coli B membrane fractions showed that phosphatidylglycerol tended to interact with membrane proteins through the mediation of Mg2+, whereas phosphatidylethanolamine had less of this tendency and was more involved in the formation of the bulk of the bilayer continuum of the membrane. These conclusions were also supported by labeling membranes with exogenous spin-labeled phospholipids, although there was some indication that exogenous phospholipids were incorporated into sites different from the sites of incorporation of phospholipids newly synthesized in situ.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 042867279

Download citation: RISBibTeXText

PMID: 6268161

DOI: 10.1016/0005-2736(81)90278-9


Related references

Covalent attachment of sulfhydryl-specific, electron spin resonance (Esr) spin-labels to Fab' fragments of murine monoclonal antibodies that recognize human platelet membrane glycoproteins. Ddevelopment of membrane protein-specific spin probes. Biochemistry 25(18): 4979-4983, 1986

Covalent attachment of sulfhydryl-specific, electron spin resonance spin-labels to Fab' fragments of murine monoclonal antibodies that recognize human platelet membrane glycoproteins. Development of membrane protein specific spin probes. Biochemistry 25(18): 4979-4983, 1986

Use of electron spin resonance spectroscopy of spin labels for studying drug-induced membrane perturbation. Journal of Pharmaceutical and Biomedical Analysis 7(6): 649-675, 1989

Combined electron-spin-resonance, X-ray-diffraction studies on phospholipid vesicles obtained from cold-hardened wheats : I. An attempt to correlate electron-spin-resonance spectral characteristics with frost resistance. Planta 170(1): 14-19, 1987

Combined electron-spin-resonance, X-ray-diffraction studies on phospholipid vesicles obtained from cold-hardened wheats: I. An attempt to correlate electron-spin-resonance spectral characteristics with frost resistance. Planta 170(1): 14-19, 1987

Electron spin echo envelope modulation and spin spin interaction studies of the iron sulphur clusters in fumarate reductase of escherichia coli. Journal of the Chemical Society Faraday Transactions 87(19): 3199-3202, 1991

Interaction of the membrane-bound D-lactate dehydrogenase of Escherichia coli with phospholipid vesicles and reconstitution of activity using a spin-labeled fatty acid as an electron acceptor: a magnetic resonance and biochemical study. Biochemistry 30(16): 3893-3898, 1991

Interaction of the membrane bound d lactate dehydrogenase of escherichia coli with phospholipid vesicles and reconstitution of activity using a spin labeled fatty acid as an electron acceptor a magnetic resonance and biochemical study. Biochemistry 30(16): 3893-3898, 1991

Membrane lysis by the antibacterial peptides cecropins B1 and B3: A spin-label electron spin resonance study on phospholipid bilayers. Biophysical Journal 77(6): 3120-3133, 1999

Spin-label electron spin resonance studies on the interactions of lysine peptides with phospholipid membranes. Biophysical Journal 73(5): 2546-2555, 1997

Phospholipid bilayer relaxation dynamics as revealed by the pulsed electron-electron double resonance of spin labels. Journal of Chemical Physics 137(14): 145102, 2013

Polarity dependence of EPR parameters for TOAC and MTSSL spin labels: correlation with DOXYL spin labels for membrane studies. Journal of Magnetic Resonance 190(2): 211-221, 2007

Investigation of very slowly tumbling spin labels by nonlinear spin response techniques: theory and experiment for stationary electron electron double resonance. Proceedings of the National Academy of Sciences of the United States of America 71(5): 1925-1929, 1974

Use of the integral of saturation transfer electron paramagnetic resonance spectra to determine molecular rotational correlation times. Slowly tumbling spin labels in the presence of rapidly tumbling spin labels. Journal of Magnetic Resonance 44(1): 109-116, 1969

Electron spin resonance determination of copper binding site on Escherichia coli cell membrane. Biofizika 41(5): 1075-1081, 1996