Effect of dietary selenium on N-2 fluorenyl-acetamide (FAA) -induced cancer in vitamin E supplemented, selenium depleted rats
Harr, J.R.; Exon, J.H.; Whanger, P.D.; Weswig, P.H.
Clinical Toxicology 5(2): 187-194
A diet low in Se and supplemented with vitamin E (NAR 40, 5330) was given from 35 days old to 80 female OSU-Brown rats 40 of which had parents which had been given a diet deficient in Se and 40 of which were from the second generation maintained on the depletion diet. FFA (N-2-fluorenyl-acetamide), a known carcinogen for rats, was added at 150 mg/kg to the diet which also had 2.5, 0.5 or 0.1 mg Se/kg or no Se added. Mammary adenocarcinomata or liver carcinomata or both occurred in 67 of the rats. From 160 to 260 days of exposure to the diets the curves of accumulative incidence of cancer were significantly higher for those given no Se or 0.1 mg/kg than for those given 0.5 or 2.5 mg Se/kg; neoplasms developed more slowly in those given 0.5 and 2.5 mg Se/kg than in the other 2 groups. Rats deprived of Se died at an earlier age than those given 0.5 and 2.5 mg/kg. Of mammary adenocarcinomata in those given Se 90% were in the thoracic region but in those given no Se 80% were in the pelvic region. Toxic hepatitis was present in 18 of 20 livers of rats given 2.5 mg Se/kg but not in any other group.