Effect of ethanol on [3H]nitrendipine binding to calcium channels in brain membranes

Greenberg, D.A.; Cooper, E.C.

Alcoholism Clinical and Experimental Research 8(6): 568-571


ISSN/ISBN: 0145-6008
PMID: 6097136
DOI: 10.1111/j.1530-0277.1984.tb05732.x
Accession: 042906658

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We examined the effect of ethanol on [3H]nitrendipine binding to rat brain membranes, to investigate the possibility that voltage-dependent calcium channels are involved in synaptic actions of ethanol. Ethanol inhibited specific [3H]nitrendipine binding with Ki = 460 mM, by decreasing binding affinity without altering the maximal number of sites labeled. At a lower concentration (100 mM), ethanol had no effect on inhibition of binding by verapamil or diltiazem. The high concentrations of ethanol required to produce alterations in [3H]nitrendipine binding suggest that ethanol and classical calcium channel antagonists influence calcium-mediated synaptic processes by separate mechanisms.