Effects of 4- (o-benzylphenoxy) -N-methylbutylamine hydrochloride (MCI-2016) on monamine metabolism in the brain
Egawa, M.; Inokuchi, T.; Ida, S.; Tobe, A.
Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica 82(5): 351-360
Effects of MCI-2016 on the uptake, contents and turnover rate of monoamines were studied in the rat brain. MCI-2016 exhibited more potent inhibitory effect on the noradrenaline (NA) uptake than on dopamine (DA) and serotonin (5-HT) uptake. Especially, the inhibitory effect of MCI-2016 on the NA uptake in the hypothalamus was comparable to that of imipramine with the IC50 value of 4 X 10(-8) M. The levels of NA and its metabolite, MHPG-SO4, in the whole brain were significantly increased by 30 mg/kg, i.p. of MCI-2016. The peak effects were reached between two to 4 hrs after administration. The increase in 5-HT contents at the cortex were also observed by MCI-2016 (30 mg/kg, i.p.), with little changes in 5-HIAA contents. The levels of DA, HVA and DOPAC in the whole brain were not significantly influenced by MCI-2016. The turnover rate of NA was facilitated by 61.1% by 15 mg/kg, i.p. of MCI-2016. DA and 5-HT turnover rates were little affected by the same dosage of MCI-2016. In the case of imipramine (15 mg/kg, i.p.), however, it didn't increase the NA turnover, and in addition, it inhibited the 5-HT turnover. The increase in NA turnover rate induced by MCI-2016 was antagonized by 54.5% by 30 mg/kg, i.p. of atropine. Physostigmine (1 mg/kg, i.p.) also increased NA turnover rate which was also partially (62.6%) inhibited by atropine. These results may suggest that the effects of MCI-2016 on noradrenergic mechanisms were qualitatively different from those of tricyclic antidepressants. In addition, the results with atropine on the turnover rate may in part suggest a possible participation of the cholinergic mechanism on the turnover increasing effect of MCI-2016.