Elemental redistribution and ultrastructural changes in guinea pig epidermis after dinitrochlorobenzene (DNCB) exposure

Lindberg, M.; Roomans, G.M.

Journal of Investigative Dermatology 81(4): 303-308

1983


ISSN/ISBN: 0022-202X
PMID: 6194230
DOI: 10.1111/1523-1747.ep12519290
Accession: 042990791

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 0-6 h
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
Energy dispersive x-ray microanalysis and transmission electron microscopy were used to study ultrastructural and chemical changes occurring in the keratinocytes at the primary irritant dinitrochlorobenzene (DNCB) reaction. The results of the microanalysis showed a similarity between dose- and time-dependent effects of DNCB and were in accordance with the ultrastructural findings. With increasing cell injury (as judged by the ultrastructure) there was an increasing loss of phosphorus, potassium, and magnesium from the cells and an increase of cellular calcium content. Sodium and chloride were only moderately changed. Results recorded when the skin was exposed to a weak DNCB dose were in accordance with a stimulation of the basal keratinocyte cell population.

Elemental redistribution and ultrastructural changes in guinea pig epidermis after dinitrochlorobenzene (DNCB) exposure