Section 44
Chapter 43,014

Enzymatic transfer of mannose from guanosine diphosphate mannose to yeast mannan-protein complexes

Bretthauer, R.K.; Tsay, G.C.

Archives of Biochemistry and Biophysics 164(1): 118-126


ISSN/ISBN: 0003-9861
PMID: 4429346
DOI: 10.1016/0003-9861(74)90013-7
Accession: 043013439

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The radioactive products derived from transfer of annose residues from GDP-annose to endogenous acceptors of a Hansenula holstii particulate enzyme preparation have been solubilized by Pronase digestion. From this soluble mixture, glycopeptides containing annose have been purified and have been shown by β-elimination-reduction experiments to contain radioactive mannose and oligosaccharides of mannose linked to serine and threonine residues.

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