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Establishment of autoantibody-producing cell lines from peripheral blood lymphocytes of patients with systemic lupus erythematosus



Establishment of autoantibody-producing cell lines from peripheral blood lymphocytes of patients with systemic lupus erythematosus



Microbiology and Immunology 24(9): 879-886



Autoantibody-producing B cell lines were established from peripheral blood lymphocytes of patients with systemic lupus erythematosus. Peripheral blood lymphocytes obtained from five of seven patients were successfully transformed by Epstein-Barr virus. Two of four established B lymphoblastoid cell lines examined in this study produced anti-nuclear factor antibodies and one of them produced anti-single-stranded DNA and anti-double-stranded DNA antibodies. These results indicate that B cell clones committed to self antigens are transformed by Epstein-Barr virus and continue to produce autoantibodies. In order to establish a monoclonal autoantibody-producing B cell line, the cells were cloned by a limiting dilution method. The data suggest that it is possible to establish a monoclonal autoantibody-producing B cell line by the combination of transformation of B cells by Epstein-Barr virus and extensive cloning.

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Accession: 043032848

Download citation: RISBibTeXText

PMID: 6971392

DOI: 10.1111/j.1348-0421.1980.tb02892.x


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