+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Estimation of insulin secretion using extracts of plasma from mice injected with anti-insulin serum: effects of arginine, glucose and anti-insulin serum



Estimation of insulin secretion using extracts of plasma from mice injected with anti-insulin serum: effects of arginine, glucose and anti-insulin serum



Journal of Endocrinology 95(1): 125-135



Anti-insulin serum (AIS) injected intravenously into adult male mice was allowed to complex endogenous plasma insulin for a fixed time before blood samples were taken. In each plasma sample, insulin was separated from antibody using acid alcohol and the free insulin was estimated by radioimmunoassay. We consider AIS to be most useful for the estimations of in-vivo insulin secretion rates over the period 0.5-5 min after its injection. The lower limit is governed by the time required for mixing and complexing of endogenous insulin. The use of a short upper limit is because antibody complexed with antigen leaves plasma more rapidly than does free antibody, carrying antigen with it. Increases in insulin per ml plasma were appreciably greater in mice injected with glucose or L-arginine plus AIS than in mice injected with glucose or L-arginine only. Hence more realistic values for in-vivo insulin secretion rates may be obtained by the use of AIS to retain most insulin in plasma than by estimation of plasma insulin levels.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 043034463

Download citation: RISBibTeXText

PMID: 6752324

DOI: 10.1677/joe.0.0950125


Related references

Insulin secretion by isolated islets in presence of glucose, insulin and anti-insulin serum. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 124(2): 497-500, 1967

Insulin secretion by isolated islets in the presence of glucose, insulin and anti-insulin serum. Proc Soc Exp Biol Med 124(2): 497-500, 1967

Glucose Metabolism In The Normal Anesthetized Dog Injected Successively With Anti-Insulin Serum And Insulin. Diabetes 13: 532-541, 1964

On Insulin 1-131 Metabolism in Mice: Modifying Effects of Anti-insulin Serum and of Total Insulin Dosage. Diabetes 15(5): 336-341, 1966

On insulin I-131 metabolism in mice. Modifying effects of anti-insulin serum and of total insulin dosage. Diabetes 15(5): 336-341, 1966

Insulin and anti-insulin effects of the plasma of healthy and sick persons. II. Semiquantitative evaluation of the insulin and anti-insulin action of plasma in Addison's disease. Revista Iberica de Endocrinologia 2(9): 329-334, 1955

Insulin biosynthesis and secretion of isolated pancreatic islets of mice after the administration of anti-insulin serum of guinea pigs. Experimental and Clinical Endocrinology 84(1): 87-90, 1984

Effects of anti-insulin serum, insulin, and glucose on output of triglycerides and on ketogenesis by the perfused rat liver. Journal of Biological Chemistry 251(1): 13-23, 1976

Effect of insulin upon the in vitro glucose utilization and glycogenesis of the diaphragm of normal and pituitarectomized rats; adaptation of this technique as an assay for serum content of insulin and anti-insulin substances. Federation Proceedings 7(1 Pt): 92, 1948

Metabolic changes by acute insulin deficiency in rabbits injected with anti-insulin serum. Acta Diabetologica Latina 6(1): 29-36, 1969

The effects of guinea pig anti insulin serum diabetes glucose and insulin on tri glyceride metabolism and ketogenesis in the isolated perfused rat liver. Federation Proceedings 29(2): 355, 1970

Evaluation of insulin secretion in response to glucose, glucagon, and arginine in a non-insulin-dependent diabetes mellitus patient with anti-GAD antibody. Journal of the Japan Diabetes Society 42(11): 937-943, 1999

Effect of pro insulin bound insulin and guinea pig anti insulin serum on the passage of insulin across isolated rat mesentery. Federation Proceedings 31(2): 255, 1972

Effects of insulin and adrenaline injected into the anterior hypothalamus on serum insulin and glucose levels. Diabetologia 13(4): 386-387, 1977

Studies on the radioimmunoassay of insulin. I. The production and detection of anti-insulin serum and anti-guinea-pig globulin rabbit serum. Eisei Shikenjo Hokoku. Bulletin of National Institute of Hygienic Sciences 88: 69-72, 1970