+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Evidence for a transcription-control region of Simian virus 40 in the adenovirus 2--Simian virus 40 hybrid, Ad2+ND 1



Evidence for a transcription-control region of Simian virus 40 in the adenovirus 2--Simian virus 40 hybrid, Ad2+ND 1



Proceedings of the National Academy of Sciences of the United States of America 69(11): 3375-3379



The complementary DNA strands of the nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid virus, Ad2(+)ND(1), were separated by isopycnic banding in a CsCl density gradient in the presence of synthetic polyribonucleotides. Separated strands were used in DNA-RNA hybridization reactions with RNA from cells productively infected by Ad2 or SV40, and with complementary SV40 RNA transcribed asymmetrically in vitro. About five times as much Ad2 RNA hybridized to the light stand of Ad2(+)ND(1) as to the heavy strand. Complementary RNA and early SV40 RNA (RNA synthesized before viral DNA replication) had significant homology only with the light strand. Only half as much of a preparation of RNA synthesized before and after viral DNA replication (early-plus-late SV40 RNA) hybridized to the light strand as to the heavy strand. These results indicate that templates for both late and early SV40 RNA are present in Ad2(+)ND(1). Therefore, the small SV40 segment within this virus (10-18% of the SV40 genome) must contain a transcription-control region. Ad2(+)ND(1) should thus be useful in the selective study of transcription as it occurs in cells infected by the oncogenic virus SV40.

(PDF emailed within 1 workday: $29.90)

Accession: 043056608

Download citation: RISBibTeXText

PMID: 4343969


Related references

Control of simian virus 40 gene expression in adenovirus-simian virus 40 hybrid viruses. Synthesis of hybrid adenovirus 2-simian virus 40 RNA molecules in cells infected with a nondefective adenovirus 2-simian virus 40 hybrid virus. Journal of Virology 13(2): 322-330, 1974

In vitro transformation by the adenovirus-simian virus 40 hybrid viruses. V. Virus-specific ribonucleic acid in cell lines transformed by the adenovirus 2-simian virus 40 and adenovirus 12-simian virus 40 transcapsidant hybrid viruses. Journal of Virology 4(5): 704-711, 1969

Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. IV. Characterization of the simian virus 40 ribonucleic acid species induced by wild-type simian virus 40 and by the nondefective hybrid virus, Ad2 + ND 1. Journal of Virology 8(2): 215-224, 1971

Simian virus 40 DNA segment of the adenovirus 7-simian virus 40 hybrid, e46, and its transcription during permissive infection of monkey kidney cells. Journal of Virology 15(5): 1214-1221, 1975

Preferred site for initiation of RNA transcription by Escherichia coli RNA polymerase within the simian virus 40 DNA segment of the nondefective adenovirus-simian virus 40 hybrid viruses Ad2 + ND 1 and Ad2 + ND 3. Journal of Virology 11(5): 682-693, 1973

Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. IX. Template topography in the early region of simian virus 40. Journal of Virology 13(3): 677-689, 1974

Studies of nondefective Adenovirus 2-Simian virus 40 hybrid viruses. 8. Association of Simian virus 40 transplantation antigen with a specific region of the early viral genome. Journal of Virology 12(4): 836-840, 1973

Simian virus 40 late promoter region able to initiate simian virus 40 early gene transcription in the absence of the simian virus 40 origin sequence. Journal of Virology 50(1): 163-173, 1984

Adenovirus type 2-simian virus 40 hybrid population: evidence for a hybrid deoxyribonucleic acid molecule and the absence of adenovirus-encapsidated circular simian virus 40 deoxyribonucleic acid. Journal of Virology 6(6): 788-794, 1970

Acylated simian virus 40-specific proteins in the plasma membrane of HeLa cells infected with adenovirus 2-simian virus 40 hybrid virus Ad2+ND2. Virology 126(2): 717-720, 1983

Genomic organization of the simian virus 40-adenovirus 7 hybrid virus, PARA(cT), that encodes a nuclear transport defective simian virus 40 T antigen. Virology 155(1): 271-276, 1986

Studies on nondefective adenovirus-simian virus 40 hybrid viruses. I. A newly characterized simian virus 40 antigen induced by the Ad2+ND 1 virus. Journal of Virology 7(2): 189-197, 1971

Efficient coupled transcription and mRNA splicing in vitro using plasmids derived from early region 3 of adenovirus 2 and a nondefective adenovirus-simian virus 40 hybrid. Proceedings of the National Academy of Sciences of the United States of America 81(9): 2679-2683, 1984

Simian virus 40-specific proteins in HeLa cells infected with nondefective adenovirus 2-simian virus 40 hybrid viruses. Journal of Virology 16(5): 1236-1247, 1975

Isolation of two plaque variants from the adenovirus type 2-simian virus 40 hybrid population which differ in their efficiency in yielding simian virus 40. Journal of Virology 5(4): 413-420, 1970