+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Expression of messenger ribonucleic acid encoding cytochrome P450 side-chain cleavage, cytochrome p450 17 alpha-hydroxylase, and cytochrome P450 aromatase in bovine follicles during the first follicular wave



Expression of messenger ribonucleic acid encoding cytochrome P450 side-chain cleavage, cytochrome p450 17 alpha-hydroxylase, and cytochrome P450 aromatase in bovine follicles during the first follicular wave



Endocrinology 136(3): 981-989



The objective of the present study was to investigate changes in the expression of messenger RNAs (mRNAs) encoding cytochrome P450 side-chain cleavage (P450scc), cytochrome P450 17 alpha-hydroxylase (P450c17), and cytochrome P450 aromatase (P450arom) at different stages of the first follicular wave of the bovine estrous cycle. Groups of heifers (three to five per group) were ovariectomized on the day of initiation of the first follicular wave (as determined by ultrasonography, day 0) or on days 2, 4, 6, 8, and 10 after initiation of the first follicular wave following estrus. Expression of mRNAs encoding P450scc, P450c17, and P450arom was detected by in situ hybridization and quantified by image analysis. P450scc mRNA was localized to theca interna cells of large preantral follicles and also to granulosa cells of follicles 4 mm or greater in diameter. mRNA for P450c17 was localized exclusively to theca interna cells, whereas P450arom mRNA was localized to granulosa cells of follicles 4 mm or greater in diameter. There were changes in mRNA levels for all three enzymes in thecal and/or granulosa cells at different times of the first follicular wave. Before identification of the dominant follicle (i.e. on days 0 and 2), there was no change in expression of P450scc and P450c17 mRNAs, whereas expression of P450arom mRNA was higher on day 2 than on day 0. Maximal mRNA levels for all three enzymes were observed on day 4. By day 6, P450scc and P450c17 mRNA levels were reduced compared to those on day 4, whereas P450arom mRNA levels remained elevated. On day 8, mRNA levels for all three enzymes were reduced. After initiation of the second follicular wave (day 10), dominant follicles from the first wave were at an advanced stage of atresia. P450scc and P450arom mRNAs were undetectable in granulosa cells, and very low levels of P450scc and P450c17 mRNAs were observed in theca interna cells. Before identification of the dominant follicle, mRNA levels for all three enzymes were similar within a cohort of follicles. Therefore, expression of these enzymes may not be associated with the mechanism of selection of the dominant follicle during a follicular wave.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 043093087

Download citation: RISBibTeXText

PMID: 7867608

DOI: 10.1210/endo.136.3.7867608


Related references

Expression of messenger ribonucleic acid encoding cytochrome P450 side- chain cleavage, cytochrome p450 17 alpha-hydroxylase, and cytochrome P450 aromatase in bovine follicles during the first follicular wave. Endocrinology 136(3): 981-989, 1995

Differentiation of bovine preovulatory follicles during the follicular phase is associated with increases in messenger ribonucleic acid for cytochrome P450 side-chain cleavage, 3 beta-hydroxysteroid dehydrogenase, and P450 17 alpha-hydroxylase, but not P450 aromatase. Endocrinology 136(11): 5102-5110, 1995

Developmental expression of cytochrome P450 side-chain cleavage and cytochrome P450 17 alpha-hydroxylase messenger ribonucleic acid and protein in the neonatal hamster ovary. Biology of Reproduction 63(6): 1586-1593, 2000

Developmental Expression of Cytochrome P450 Side-Chain Cleavage and Cytochrome P450 17-Hydroxylase Messenger Ribonucleic Acid and Protein in the Neonatal Hamster Ovary. Biology of Reproduction 63(6): 1586-1593, 2000

Increased expression of messenger ribonucleic acid encoding cytochrome P450 cholesterol side-chain cleavage and P450 17alpha-hydroxylase enzymes in ovarian hyperthecosis. Fertility and Sterility 71(2): 328-333, 1999

Changes in messenger ribonucleic acid encoding luteinizing hormone receptor, cytochrome P450-side chain cleavage, and aromatase are associated with recruitment and selection of bovine ovarian follicles. Biology of Reproduction 56(5): 1158-1168, 1997

Levels of messenger ribonucleic acid for cytochrome P450 17 alpha-hydroxylase and P450 aromatase in preovulatory bovine follicles decrease after the luteinizing hormone surge. Endocrinology 132(5): 2239-2245, 1993

Expression of cytochrome P-450 side chain cleavage , cytochrome P-450 17-alpha-hydroxylase and cytochrome P-450 aromatase mRNAs in bovine follicles during the first follicular wave. Biology of Reproduction 50(Suppl. 1): 162, 1994

Multiple mechanisms for regulation of 3 beta-hydroxysteroid dehydrogenase/delta 5----delta 4-isomerase, 17 alpha-hydroxylase/C17-20 lyase cytochrome P450, and cholesterol side-chain cleavage cytochrome P450 messenger ribonucleic acid levels in primary cultures of mouse Leydig cells. Endocrinology 129(3): 1429-1435, 1991

Human cultured adrenal fasciculata-reticularis cells are targets for angiotensin-II: effects on cytochrome P450 cholesterol side-chain cleavage, cytochrome P450 17 alpha-hydroxylase, and 3 beta-hydroxysteroid-dehydrogenase messenger ribonucleic acid and proteins and on steroidogenic responsiveness to corticotropin and angiotensin-II. Journal of Clinical Endocrinology and Metabolism 78(5): 1212-1219, 1994

Insulin and IGF-I are necessary for FSH-induced cytochrome P450 aromatase but not cytochrome P450 side-chain cleavage gene expression in oestrogenic bovine granulosa cells in vitro. Journal of Endocrinology 174(3): 499-507, 2002

Levels of messenger ribonucleic acid encoding cholesterol side-chain cleavage cytochrome P-450, 17 alpha-hydroxylase cytochrome P-450, adrenodoxin, and low density lipoprotein receptor in bovine follicles and corpora lutea throughout the ovarian cycle. Molecular Endocrinology 1(3): 274-279, 1987

Modulation of ovarian cytochrome P450 17 alpha-hydroxylase and cytochrome aromatase messenger ribonucleic acid by prolactin in the domestic turkey. Biology of Reproduction 52(3): 600-608, 1995

Detection of aromatase cytochrome P450, 17 alpha-hydroxylase cytochrome P450 and NADPH:P450 reductase on the surface of cells in which they are expressed. Molecular and Cellular Endocrinology 119(1): 69-74, 1996

Aromatase cytochrome P450 and cholesterol side-chain cleavage cytochrome P450 in corpora lutea of pregnant rats: diverse regulation by peptide and steroid hormones. Endocrinology 125(3): 1673-1682, 1989